Formula and preparation process of culture medium for symbiosis of bacteria and plants

A culture substrate and nutrient substrate technology, applied in the field of bacteria and plant symbiosis culture substrate formula and preparation technology, can solve the problems of poor growth and development of plants, failure to meet the needs of bacteria and plants symbiosis, and failure to guarantee

Pending Publication Date: 2021-12-21
GUANGDONG OCEAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to provide a culture substrate formula and preparation process for the symbiosis of bacteria and plants, so as to solve the problem that the bacteria and their metabolites proposed in the above background technology are likely to have adverse effects on the growth and development of associated plants during the production process In severe cases, both sides will die out at the same time, thus unable to meet the symbiotic needs of bacteria and plants, resulting in strict growth conditions for the environment, and at the same time leading to the problem that the production of plants cannot be guaranteed.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0050] Each Kg of bacteria and plant symbiotic culture medium contains:

[0051] Phosphoric acid 5g, potassium sulfate 5g, zinc sulfate 5g, urea 10g, lignin calcium benzenesulfonate 10g, sodium chloride 10g, ammonia water 10g, agricultural amino acid 20g, lignite 20g, medical stone 20g, zeolite powder 20g, phosphogypsum 40g, 40g of humus and 40g of diatomaceous earth.

[0052] The above joint recipes can obtain the symbiotic nutrient matrix for bacteria and plants, with a pH value of 5.0-7.5 (depending on the pH value of the soil to be used)

[0053] A preparation process of a bacterium-plant symbiotic culture substrate formula, comprising the following steps:

[0054] S1: Add 1g-100g of ammonia water per Kg of bacteria-plant symbiotic nutrient matrix to 10g-400g of lignite;

[0055] a. Stir evenly;

[0056] b. Dry both sides;

[0057] c. Grinding and crushing treatment;

[0058] S2: Add 1g-100g of urea per Kg of bacteria and plant symbiotic nutrient matrix to 10g-400g of...

Embodiment 2

[0069] Each Kg of bacteria and plant symbiotic culture medium contains:

[0070] Phosphoric acid 10g, potassium sulfate 10g, zinc sulfate 10g, urea 20g, calcium lignin benzenesulfonate 20g, sodium chloride 20g, ammonia water 20g, agricultural amino acid 40g, lignite 40g, medical stone 40g, zeolite powder 40g, phosphogypsum 80g, 80g of humus and 80g of diatomaceous earth.

[0071] The above joint recipes can obtain the symbiotic nutrient matrix for bacteria and plants, with a pH value of 5.0-7.5 (depending on the pH value of the soil to be used)

[0072] A preparation process of a bacterium-plant symbiotic culture substrate formula, comprising the following steps:

[0073] S1: Add 1g-100g of ammonia water per Kg of bacteria-plant symbiotic nutrient matrix to 10g-400g of lignite;

[0074] a. Stir evenly;

[0075] b. Dry both sides;

[0076] c. Grinding and crushing treatment;

[0077] S2: Add 1g-100g of urea per Kg of bacteria and plant symbiotic nutrient matrix to 10g-400g...

Embodiment 3

[0088] Each Kg of bacteria and plant symbiotic culture medium contains:

[0089] Phosphoric acid 100g, potassium sulfate 100g, zinc sulfate 100g, urea 100g, calcium lignin benzenesulfonate 100g, sodium chloride 100g, ammonia water 100g, agricultural amino acid 100g, lignite 400g, medical stone 400g, zeolite powder 400g, phosphogypsum 400g, 400g of humus and 400g of diatomaceous earth.

[0090] The above joint recipes can obtain the symbiotic nutrient matrix for bacteria and plants, with a pH value of 5.0-7.5 (depending on the pH value of the soil to be used)

[0091] A preparation process of a bacterium-plant symbiotic culture substrate formula, comprising the following steps:

[0092] S1: Add 1g-100g of ammonia water per Kg of bacteria-plant symbiotic nutrient matrix to 10g-400g of lignite;

[0093] a. Stir evenly;

[0094] b. Dry both sides;

[0095] c. Grinding and crushing treatment;

[0096] S2: Add 1g-100g of urea per Kg of bacteria and plant symbiotic nutrient matr...

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Abstract

The invention discloses a formula and a preparation process of a culture medium for symbiosis of bacteria and plants. Every Kg of the culture medium for symbiosis of bacteria and plants contains 1 to 100 g of phosphoric acid, 1 to 100 g of potassium sulfate, 1 to 100 g of zinc sulfate, 1 to 100 g of urea, 1 to 100 g of lignin calcium benzenesulfonate, 1 to 100 g of sodium chloride, 1 to 100 g of ammonia water, 1 to 100 g of agricultural amino acid, 10 to 400 g of lignite, 10 to 400 g of medical stone, 10 to 400 g of zeolite powder, 10 to 400 g of ardealite, 10 to 400 g of humus soil and 10 to 400 g of diatomite, the above components are combined to obtain the bacteria and plant symbiotic nutrient medium, and the pH value is 5.0-7.5 (according to the PH value of soil to be applied). According to the formula and the preparation process of the culture medium for symbiosis of the bacteria and the plants, nutrient substances necessary for growth of the bacteria and the plants are added, and the co-culture medium capable of simultaneously culturing the bacteria and the plants and researching the influence of the bacteria and metabolites thereof on growth and development of the plants is prepared, so that symbiosis requirements of the bacteria and the plants are met, the growth quality of the plants is improved, and the growth conditions are reduced.

Description

technical field [0001] The invention relates to the technical field of bacteria and plants symbiosis, in particular to a culture substrate formula and preparation process for bacteria and plants symbiosis. Background technique [0002] Plants are one of the more important species in nature, and they have strict requirements on the growth environment. In the environment where bacteria and other substances exist, the growth of plants will also be affected. Therefore, the association between plants and bacteria has always been one of the research points that people pay more attention to; [0003] During the production process of bacteria and their metabolites, it is easy to have adverse effects on the growth and development of associated plants. In severe cases, both parties will die out simultaneously, thus failing to meet the symbiosis needs of bacteria and plants, resulting in strict environmental growth conditions. , resulting in a decline in the yield of the plant cannot ...

Claims

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Application Information

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IPC IPC(8): A01H4/00C05G3/00C12N1/20
CPCA01H4/002A01H4/00C05B15/00C05G3/00C12N1/20C05C11/00C05D1/02C05D3/02C05D9/00C05D9/02C05F11/02
Inventor 郑殿峰冯乃杰饶刚顺冯胜杰孟枫岩刘美玲
Owner GUANGDONG OCEAN UNIVERSITY
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