Bio-ink for 3D bio-printing and preparation method and application thereof

A bio-ink and bio-printing technology, applied in tissue regeneration, prosthesis, additive processing, etc., can solve the problems of high cost and poor safety, and achieve the effect of high safety, good flexibility and high proliferation ability

Active Publication Date: 2021-12-28
GUANGDONG VITALIFE BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Existing bio-inks often need to add photosensitive resin, the cost of one is high, and the safety of the two is relatively poor

Method used

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  • Bio-ink for 3D bio-printing and preparation method and application thereof
  • Bio-ink for 3D bio-printing and preparation method and application thereof
  • Bio-ink for 3D bio-printing and preparation method and application thereof

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preparation example Construction

[0080] Correspondingly, the present invention also discloses a preparation method of the above-mentioned bio-ink, which includes the following steps:

[0081] (1) preparing biologically active cells;

[0082] (2) Take 0.2-1 part of sodium alginate, 0.3-1 part of gelatin, 0.15-0.5 part of cellulose and 120-150 parts of buffer solution, mix well to obtain a hydrogel;

[0083] Specifically, in one embodiment of the present invention, sodium alginate, gelatin, cellulose, and buffer are mixed simultaneously. In another embodiment of the present invention, sodium alginate, gelatin, cellulose and part of the buffer are mixed sequentially, and then the resulting solution is fully mixed to obtain a hydrogel.

[0084] Specifically, in one embodiment of the present invention, the bioactive cells are suspended in the hydrogel to obtain the bio-ink body; and then the bio-ink body is printed. In another embodiment of the present invention, the hydrogel and the bio-ink are provided separat...

Embodiment 1

[0103] This embodiment provides a bioink, which includes hydrogel, bioactive cells and a curing agent solution;

[0104] Wherein, the hydrogel comprises the following components by weight percentage:

[0105] 0.3 parts of sodium alginate, 0.35 parts of gelatin, 0.15 parts of nanocellulose, and 125 parts of 1×PBS buffer.

[0106] Curing agent solution comprises the following components by weight percentage:

[0107] CaCl 2 5 parts, 95 parts of 1×PBS buffer.

[0108] The bioactive cells in the bioink are amniotic fluid mesenchymal stem cells (AFS cells), and the number is 1.7 × 10 7 individual / mL.

[0109] The preparation method of bioink is:

[0110] (1) preparing amniotic fluid mesenchymal stem cells;

[0111] For details, see patent CN201811148883.5.

[0112] (2) prepare hydrogel, specifically as follows:

[0113] 1) Weigh 300mg of sodium alginate powder and dissolve it in 100ml of phosphate-buffered saline (PBS), put it into a magnet and stir it in a water bath at 4...

Embodiment 2

[0120] This embodiment provides a bioink, which differs from that of Embodiment 1 in that human skin fibroblasts (Fbs cells) are used as bioactive cells, and its specific preparation method can be found in patent CN201910777323.4.

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Abstract

The invention discloses bio-ink for 3D bio-printing. The bio-ink comprises hydrogel, bioactive cells and a curing agent solution; the hydrogel is prepared from the following components including, by weight, 0.2-1 part of sodium alginate, 0.3-1 part of gelatin, 0.15-0.5 part of cellulose and 120-150 parts of a buffer solution; the number of the bioactive cells is 1*10<7>/mL-2*10<7>/mL; and the curing agent solution is prepared from the following components including, by weight, 2-10 parts of CaCl2 and 90-98 parts of a buffer solution. The invention also discloses a preparation method and application of the bio-ink, and further comprises a printing method based on the bio-ink. By implementing the preparation method disclosed by the invention, the bio-ink with controllable biodegradability, mechanical strength and drug loading performance can be obtained.

Description

technical field [0001] The invention relates to the technical field of bio-ink, in particular to a bio-ink for 3D bio-printing and its preparation method and application. Background technique [0002] The skin plays a vital role as the largest tissue in the human body, and severe skin injuries caused by burns or trauma often fail to heal on their own and require the use of skin tissue substitutes. Generally, autologous layered skin grafting is used clinically, but if the wound is extensive, the number and size of the donor site are limited, which cannot meet the clinical application. On the other hand, although allogeneic transplantation can avoid insufficient sources, it often has high immune rejection and a relatively low success rate. [0003] The cellularized graft-like product is a product of the rapid development of tissue engineering, which is a scaffold patch provided in the form of polymer materials combined with human fibroblasts. Efficacy has improved but is exp...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/60A61L27/54A61L27/50A61L27/38A61L27/20A61L27/22B33Y70/10B33Y80/00B33Y10/00
CPCA61L27/60A61L27/54A61L27/50A61L27/3834A61L27/3804A61L27/20A61L27/222B33Y70/10B33Y80/00B33Y10/00A61L2430/40C08L5/04C08L1/02
Inventor 唐淑艳刘小翠蒙燕瑶杨景利许峻荣
Owner GUANGDONG VITALIFE BIOTECHNOLOGY CO LTD
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