Injectable self-healing antibacterial hydrogel dressing preloaded with polypeptide and preparation method thereof

A hydrogel, self-healing technology, applied in the field of medical materials and its preparation, can solve the problems of unfavorable wound repair, damage, lack of self-healing ability of injectable hydrogel, etc., and achieve excellent biocompatibility and biodegradability , improve safety, and accelerate wound healing

Active Publication Date: 2021-09-07
DONGHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] During clinical treatment, especially for irregular wound sites and deep wound sites, conventional injectable hydrogels are easily damaged by external forces due to the lack of self-healing ability, which is not conducive to wound repair

Method used

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  • Injectable self-healing antibacterial hydrogel dressing preloaded with polypeptide and preparation method thereof
  • Injectable self-healing antibacterial hydrogel dressing preloaded with polypeptide and preparation method thereof
  • Injectable self-healing antibacterial hydrogel dressing preloaded with polypeptide and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] A preparation method of an injectable self-repairing antibacterial hydrogel dressing preloaded with polypeptide:

[0046] a) Preparation of aldylated dextran:

[0047] At room temperature, the number average molecular weight of 10000 dextran ( figure 1Structural formula 1) (6g) was dissolved in 100mL deionized water, sodium periodate (2g) was dissolved in 5mL deionized water, sodium periodate solution was added dropwise to the dextran solution, and the reaction was carried out under dark conditions 6 hours, add ethylene glycol (2g) room temperature reaction 1 hour again, gained product ( figure 1 Structural formula 2) was dialyzed for one week, freeze-dried, and stored at low temperature.

[0048] b) Preparation of modified dextran:

[0049] At room temperature, dissolve aldylated dextran (1 g) in deionized water, dissolve dodecylamine (0.1 g) in ethanol solution, and dissolve aldylated dextran ( figure 1 In structural formula 2) solution, add dodecylamine ( figur...

Embodiment 2

[0066] A preparation method of an injectable self-repairing antibacterial hydrogel dressing preloaded with polypeptides differs from Example 1 in that:

[0067] Described step a) is:

[0068] a) Preparation of aldylated dextran:

[0069] Under room temperature conditions, the number average molecular weight is 50000 dextran ( figure 1 Structural formula 1) (6g) was dissolved in 100mL deionized water, sodium periodate (2g) was dissolved in 5mL deionized water, sodium periodate solution was added dropwise to the dextran solution, and the reaction was carried out under dark conditions 6 hours, add ethylene glycol (2g) room temperature reaction 1 hour again, gained product ( figure 1 Structural formula 2) was dialyzed for one week, freeze-dried, and stored at low temperature.

[0070] b) Preparation of modified dextran:

[0071] At room temperature, dissolve aldylated dextran (1 g) in deionized water, dissolve dodecylamine (0.1 g) in ethanol solution, and dissolve aldylated de...

Embodiment 3

[0073] A preparation method of an injectable self-repairing antibacterial hydrogel dressing preloaded with polypeptides differs from Example 1 in that:

[0074] Described step a) is:

[0075] a) Preparation of aldylated dextran:

[0076] Under room temperature conditions, the number average molecular weight is 100000 dextran ( figure 1 Structural formula 1) (6g) was dissolved in 100mL deionized water, sodium periodate (2g) was dissolved in 5mL deionized water, sodium periodate solution was added dropwise to the dextran solution, and the reaction was carried out under dark conditions 6 hours, add ethylene glycol (2g) room temperature reaction 1 hour again, gained product ( figure 1 Structural formula 2) was dialyzed for one week, freeze-dried, and stored at low temperature.

[0077] b) Preparation of modified dextran:

[0078] At room temperature, dissolve aldylated dextran (1 g) in deionized water, dissolve dodecylamine (0.1 g) in ethanol solution, and dissolve aldylated d...

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Abstract

The invention discloses an injectable self-repairing antibacterial hydrogel dressing of preloaded polypeptide and a preparation method thereof. The hydrogel dressing includes hydrogel combined with fabric, and polypeptides promoting fibroblast migration are distributed on the surface and inside of the hydrogel. The preparation method is as follows: dextran with different molecular weights is selectively oxidized by an oxidant to form an aldylated dextran; a partially alkylated dextran is obtained by reacting an aldylated dextran with aliphatic amine; and then the polypeptide is prepared in a solid phase ; Formaldehyde dextran, adipate dihydrazide and partially alkylated dextran were dissolved in PBS buffer respectively, and the polypeptide was added to the formaldehyde dextran solution, pre-loaded with solid-phase synthesized For the polypeptide, the three solutions are mixed according to a certain mass ratio to prepare an injectable self-repairing antibacterial hydrogel of the preloaded polypeptide, which is finally made into a dressing. The product prepared by the invention has good antibacterial performance, can reduce the chance of wound infection, and its dynamic self-repair performance can target irregular wounds, and can also keep the wound surface moist and accelerate wound healing.

Description

technical field [0001] The invention relates to an injectable dynamic self-repairing antibacterial hydrogel dressing preloaded with polypeptide and a preparation method thereof, belonging to the technical field of medical materials and its preparation. Background technique [0002] Wound healing is a complex and coordinated process that includes hemostasis, inflammation, cell proliferation and extracellular matrix remodeling. Currently, bacterial infection and poor wound healing remain major challenges in wound management. Bacterial infection often leads to the formation of exudate, which greatly hinders wound healing. In addition, chronic and deep wounds are often difficult to angiogenesis and self-repair due to reduced activity of endogenous growth factors. To overcome these problems, many wound dressing materials such as films, hydrogels, foams, and hydrocolloids have been developed to meet the requirements of preventing infection and accelerating wound closure. Among ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L15/28A61L15/32A61L15/42A61L15/46A61L15/44A61L15/62A61L26/00C07K14/47C07K1/06C07K1/04
CPCA61L15/28A61L15/32A61L15/42A61L15/44A61L15/46A61L15/62A61L26/0023A61L26/0047A61L26/0066A61L26/008A61L26/009A61L2300/232A61L2300/252A61L2300/404A61L2300/412A61L2400/06C07K14/47C08L5/02C08L89/00Y02P20/55
Inventor 吴德群叶婷婷刘畅王学利李发学俞建勇
Owner DONGHUA UNIV
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