Biological crust restoration material for promoting ecological restoration of ionic rare earth tailing area, application and restoration method
An ionic rare earth and biological crusting technology, applied in the field of mine ecological restoration, can solve the problems of soil degradation, environmental pollution in mining areas, and reduction of exposed surface layers, etc.
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[0044] In the present invention, the preparation method of the algae suspension preferably comprises the following steps:
[0045] Inoculate the biological crust restoration material in the ion-promoting rare earth tailings area into the culture solution, and cultivate to obtain the algae suspension.
[0046] In the present invention, the nutrient solution is preferably a sterile BG-11 nutrient solution; the composition of the sterilized BG-11 nutrient solution is as shown in Table 1; For special limitations, it is enough to prepare the mother solution according to the formula in Table 1 according to the process well known in the art and mix to form the BG-11 culture solution.
[0047] Table 1 Composition and preparation method of BG-11 culture medium
[0048]
[0049] Note: A5 solution (diluted to 1000mL with water): H 3 BO 3 (61.0 mg); MnSO 4 ·H 2 O (169.0 mg); ZnSO 4 ·7H 2 O (287.0 mg); CuSO 4 ·5H 2 O (2.5mg); (NH 4 )Mo 7 o 24 4H 2 O (12.5 mg).
[0050] Unl...
Embodiment 1
[0055] The biocrust samples formed under natural conditions in the abandoned rare earth mining area of Daluoshi, Wenfeng Town, Xunwu County, Ganzhou City, Jiangxi Province were collected and sterilized with 75% alcohol to prevent cross-contamination of the samples during the collection process. The sterilized samples were Put it in a sterile aluminum box (specification: 50mm×30mm), transport it back to the laboratory, and store it at a low temperature of -4°C; In the 150mL Erlenmeyer flask of -11 culture fluid (composition and preparation method of BG-11 culture fluid are shown in Table 1), after being uniformly dispersed in the homogenizer, it is cultured statically under specific environmental conditions in the light incubator (light and dark Ratio = 16h: 8h, light intensity at 3000lx, temperature at 25°C), after 4 weeks of cultivation, when an obvious algae solution is formed, use a capillary to take 2 μL of the algae solution into a 24-well cell culture containing 2mL of ...
Embodiment 2
[0058] The biocrust samples formed under natural conditions in the abandoned rare earth mining area of Daluoshi, Wenfeng Town, Xunwu County, Ganzhou City, Jiangxi Province were collected and sterilized with 75% alcohol to prevent cross-contamination of the samples during the collection process. The sterilized samples were Put it in a sterile aluminum box (specification: 50mm×30mm), transport it back to the laboratory, and store it at a low temperature of -4°C; In the 150mL Erlenmeyer flask of -11 culture fluid (composition and preparation method of BG-11 culture fluid are shown in Table 1), after being uniformly dispersed in the homogenizer, it is cultured statically under specific environmental conditions in the light incubator (light and dark Ratio = 16h: 8h, light intensity at 3000lx, temperature at 25°C), after 4 weeks of cultivation, when an obvious algae solution is formed, use a capillary to take 2 μL of the algae solution into a 24-well cell culture containing 2mL of ...
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