Microscopic image acquisition control method, control system and computer readable storage medium

Abstract

The invention discloses a rapid and accurate focusing microscopic image acquisition control method. The method comprises the following steps: S1, acquiring plane coordinate information of a reference point and at least two pre-selected points from a currently acquired glass slide sample image; s2, controlling the objective lens to perform full-range focusing on the reference point on the glass slide sample along the Z-axis direction to obtain initial coordinate information after focusing; s3, controlling the objective lens to adjust and focus the reference point and the at least two pre-selected points on the glass slide sample in the Z-axis direction according to Z-axis information in the plane coordinate information and the initial coordinate information, and obtaining at least three pieces of adjustment coordinate information; and S4, constructing a fitting plane of the glass slide sample according to the at least three pieces of adjustment coordinate information, and then carrying out image scanning on the glass slide sample on the fitting plane so that the sample image can be rapidly and accurately scanned.

Description

technical field [0001] The invention relates to the field of microphotography, in particular to a fast and precise focusing microscopic image acquisition control method, control system and computer-readable storage medium. Background technique [0002] Pathological sectioning is to process some diseased tissues or organs through various chemicals and embedding methods to fix and harden them, cut them into thin slices on a microtome, adhere to glass slides, and dye them in various colors for examination under a microscope. To observe pathological changes, make pathological diagnosis, and provide assistance for clinical diagnosis and treatment. With the advancement of science and technology, people combine microscopes with scanners, and the microscopic images of pathological sections are displayed on the display screen, which is convenient for doctors to view . [0003] In order to improve detection efficiency, most institutions have adopted automated image acquisition method...

AI Technical Summary

Problems solved by technology

Although the existing automatic image acquisition method can collect images, which improves the accuracy and efficiency of detection, however, in the actual microscopic shooting process, the focusing process is too long, and the focus often occurs Therefore, fast and accurate focusing on the sample image is still the key point that needs continuous improvement. The quality of the focus directly affects the success or failure of the sample image scanning, and it will also cause great interference to the detection results.

[0004] In view of this, it is necessary to provide a fast and precise focusing microscopic image acquisition control method to solve or at least alleviate the above-mentioned defects of slow focusing process and unclear focus

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