Fluorescent quantitative detection reagent for cat cystatin C and preparation method thereof

Abstract

The invention provides a fluorescent quantitative detection reagent for cat cystatin C and a preparation method thereof, and relates to the field of biological detection. The fluorescent quantitative detection reagent for the cat cystatin C comprises the following raw materials: a treating fluid, a glass fiber membrane, an MES buffer solution, microspheres, EDC-HCl, NHS, a sealing agent and a marking diluent. The preparation method comprises the following steps: S1, (1) preparing the following solutions by using 10 mM of PBS: 3% of BSA, 3% of sucrose, 1% of PVP-K30, 1% of casein, 1% of PEG20000, 0.1% of Triton X-100 and 0.02% of NaN3; and (2) at room temperature, soaking a glass fiber membrane with the treating fluid for 12 hours; S2, diluting 0.25 mL of 1 [mu]mol / L microspheres with 0.25 ml of 20 mmol / L MES buffer solution to a concentration of 0.5 [mu]mol / L; S3, adding 5 [mu]l of EDC-HCl with a concentration of 20 mg / ml and 5 [mu]l of NHS with a concentration of 20 mg / ml into the MES buffer solution; S4, after activating the microspheres, immediately adding 0.1 mg of to-be-labeled antibody; S5, adding 25 [mu]l of a sealing agent, and re-suspending and dispersing by using 2 ml of a marking diluent; and S6, spraying a combination pad. According to the invention, by improving the sensitivity of reagent testing and prolonging the validity period of the reagent, industrial production is facilitated.

Description

Technical field [0001] The present invention relates to the field of biological detection, in particular a carbocystin C fluorescence quantitative detection reagent and a preparation method thereof. Background technique [0002] Early diagnosis of renal disease has always been concerned, evaluating the gold standard of dog cat renal function is a glomerular filtration rate (GFR), which is generally measured by jewery or iodo-hexol test, but this method is technique for clinical technology. High, difficulty in operation, therefore, in the clinic of veterinarians, the serum urea nitrogen (CREA) is often evaluated by serum urea nitrogen (CREA), and the concentration of serum urea nitrogen and serum creatinine (CREA) is used. Among many other factors, such as feeding state, body function, weight, muscle amount, etc. In addition, when the glomerular filtration rate (GRF) is reduced by 75%, serum creatinine concentration will increase significantly, so people have never stopped on the ...

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