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Cervical cell preservation solution

A cervical cell and preservation solution technology, applied in the field of cell preservation, can solve the problems of diagnosis impact, health threat to testing personnel, environmental pollution of cell preservation solution, etc., and achieve the effect of low cost

Inactive Publication Date: 2022-01-28
武汉中纪生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, the cell preservation solution on the market pollutes the absolute environment and poses a certain threat to the health of the testing personnel; the cell shape is not fixed well, and the shape of the nucleus and cytoplasm can

Method used

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  • Cervical cell preservation solution
  • Cervical cell preservation solution
  • Cervical cell preservation solution

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0059] Add 400g of ethanol, 21.85g of disodium hydrogen phosphate, 6.09g of sodium dihydrogen phosphate, 5g of acetic acid, 2g of alkylphenol polyoxyethylene ether, 9g of sodium chloride, 2g of sodium edetate and 1.3g of calcium chloride 500g of pure water, after all substances are completely dissolved, continue to add pure water to 1000g to obtain the cervical cell preservation solution; use sodium hydroxide to adjust the pH of the cell preservation solution to 7. The cell preservation solution has good antibacterial properties, no microbial growth, good cell shape maintenance, no red precipitate caused by blood coagulation, and no cell aggregation caused by mucus.

Embodiment 2

[0061] Add 200g of methanol, 10g of HEPES, 1g of acetic acid, 0.1g of long-chain fatty alcohol polyoxyethylene ether, 1g of sodium chloride, 1g of sodium citrate and 0.21g of copper chloride into 300g of pure water in turn, until all substances are completely dissolved After that, continue to add pure water to 1000g to obtain the cervical cell preservation solution; adjust the pH of the cell preservation solution to 6 with hydrochloric acid. The cell preservation solution has good antibacterial properties, no microbial growth, good cell shape maintenance, no red precipitation caused by blood coagulation, and no cervical cell aggregation.

Embodiment 3

[0063] Add 500g of isopropanol, 36g of potassium dihydrogen phosphate, 14g of sodium hydroxide, 10g of acetic acid, 5g of polyoxyethylene fatty acid ester, 10g of sodium chloride, 10g of sodium heparin and 2.1g of zinc chloride into 380g of pure water in turn, and wait until all After the substances are completely dissolved, continue to add pure water to 1000 g to obtain the cervical cell preservation solution; adjust the pH of the cell preservation solution to 8 with sodium hydroxide. The cell preservation solution has good antibacterial properties, no microbial growth, good cell shape maintenance, no red precipitate caused by blood coagulation, and no cell aggregation caused by mucus.

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Abstract

The invention relates to the technical field of cell preservation, in particular to a cervical cell preservation solution which comprises an alcohol solution, a buffer solution, an acid solution, a surfactant, sodium chloride, an anticoagulant and metal ions. The cell preservation solution does not contain components harmful to a human body, and is safe and environment-friendly; the cell preservation solution has good treatment capacity on samples containing blood and mucus; protein in cervical exfoliated cells is denatured, cell activity is terminated, and the cell morphology can be kept in a sampling state; corrosion prevention and sterilization can be achieved, and it is guaranteed that cervical cells stored in the cell preservation solution are not decomposed by foreign microorganisms; and the cells can be preserved for a longer time.

Description

technical field [0001] The invention relates to the technical field of cell preservation, and more specifically, to a cervical cell preservation solution. Background technique [0002] Both Pap smear and TCT are cytological examinations for cervical exfoliated cells. The Pap smear method refers to scraping the exfoliated cells of the cervix through the vaginal speculum, and observing whether the cells are abnormal under a microscope. The Pap smear method is cheap and convenient for census. Since its introduction in 1941, it has been widely used in cervical cancer screening and has made important contributions to reducing the incidence and mortality of cervical cancer. The Pap smear method is relatively cheap and convenient for census, but the disadvantage is that the specimen cells prepared by this method are piled up together, which is not convenient for observation, the diagnostic accuracy is low, and there is a certain rate of misdiagnosis and missed diagnosis. TCT tech...

Claims

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Application Information

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IPC IPC(8): A01N1/02
CPCA01N1/021A01N1/0221A01N1/0226A01N1/0215
Inventor 张珺邹亚平狄媛媛胡鹏高
Owner 武汉中纪生物科技有限公司