SNP molecular marker for screening Gansu nectarine varieties

A molecular marker and nectarine technology, which is applied in the direction of recombinant DNA technology, microbiological determination/inspection, DNA/RNA fragments, etc., can solve the problems of undeveloped molecular markers and inability to distinguish Gansu nectarine varieties, so as to achieve good application and promotion The effect of prospects, few restrictions, and simple methods

Pending Publication Date: 2022-01-28
SHANDONG AGRICULTURAL UNIVERSITY
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Problems solved by technology

Among the nectarine varieties, Gansu nectarine varieties are relatively high-quality nectarine germplasm resources. However, so far, molecular markers closely linked to Gansu nectarines have not been developed, and Gansu nectarine varieties cannot be distinguished. Screening of high-quality germplasm resources

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  • SNP molecular marker for screening Gansu nectarine varieties
  • SNP molecular marker for screening Gansu nectarine varieties
  • SNP molecular marker for screening Gansu nectarine varieties

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Embodiment 1

[0029] 1. Development of Gansu Nectarine Gene Markers in Natural Variety Populations

[0030] In order to solve the problem of obtaining accurate phenotype-associated sites, the present invention combined the high-depth resequencing data (average depth 20.3X) of 417 cultivated peach varieties covering each major production area for variation (SNPs) detection, and screened 3,749,618 SNPs sites , using genome-wide association analysis (GWAS), it was found that a SNP located at 15,893,290 bp of chromosome Pp05 in the peach genome showed a strong correlation with Gansu nectarine, named FH-SNP-15.89, as figure 1 The position in the box in .

[0031] figure 2 It is the phylogenetic tree, PCA, population structure and LD reduction trend of 417 peach resources, figure 2 A in a is the high-quality SNP variation data filtered based on the whole genome, and the phylogenetic tree constructed based on the proximity method, figure 2 b in b is the PCA plot of the first 2 component prin...

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Abstract

The invention discloses an SNP molecular marker for screening Gansu nectarine varieties. Gansu nectarine genes are finely positioned, the stable, co-dominant and polymorphic SNP molecular marker is developed in a fine positioning area, the SNP molecular marker can be used for screening or identifying Gansu nectarine varieties, early screening of high-quality germplasm resources is facilitated, a foundation is laid for fine positioning and molecular cloning of the Gansu nectarine genes, and the SNP molecular marker can be used for screening or identifying the Gansu nectarine varieties. Meanwhile, an efficient way is provided for molecular marker-assisted breeding of a new peach variety with the Gansu nectarine gene.

Description

technical field [0001] The invention relates to a SNP molecular marker for screening Gansu nectarine varieties, and belongs to the technical field of molecular marker-assisted selection. Background technique [0002] Peach is one of the most economically important fruit tree species in the world, diploid (2n=16), due to its small genome (~250M) and relatively short childhood (2~3 years), it is regarded as a key factor in the evolution of Rosaceae. A valuable resource and an important model plant for functional genomics research (Shulaev et al., 2008). Peach is native to China, domesticated in China 4,000 years ago, and then passed to European countries through the Silk Road (Faust et al., 1995). In my country, peach is the third largest deciduous fruit tree, which is widely cultivated all over the country and is an important part of agricultural production. [0003] Glabrous trait of peach peel affects fruit appearance quality and ecological adaptability. Nectarines are w...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6895C12Q1/6858C12N15/11
CPCC12Q1/6895C12Q1/6858C12Q2600/13C12Q2600/156C12Q2531/113C12Q2565/125
Inventor 李玲肖伟谭秋平
Owner SHANDONG AGRICULTURAL UNIVERSITY
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