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Method for separating biomolecules

A technology for biomolecules and separation equipment, applied in separation methods, biochemical equipment and methods, and peptide preparation methods, etc.

Pending Publication Date: 2022-01-28
CYTIVA SWEDEN AB
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] It is an object of the present disclosure to provide an improved method for periodically removing harvest from a bioreactor and for separation which solves or at least alleviates the problems described above

Method used

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  • Method for separating biomolecules
  • Method for separating biomolecules
  • Method for separating biomolecules

Examples

Experimental program
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example 1

[0250] The typical length of a priming process run of about one month matches well with the typical lifetime of a continuous cycle column run in batch mode. By way of non-limiting example, assuming that the chromatography resin lasts 100 cycles and the perfusion process runs for 25 days, the flow rates of the chromatography steps can be designed such that each cycle of the chromatography steps lasts 6 hours, i.e. 4 cycles per 24 hours, and during the perfusion run 100 cycles. This allows resin life to be fully utilized while removing the need to replace columns during perfusion runs or store columns after runs. Thus, true single-use resin chromatography is achieved in a cost-effective manner. For example, each cycle of 6 hours can be divided into 4 hours of column loading and 2 hours of column washing, elution, and regeneration. In this example, if the reactor volume per day (RV / day) is set to 1 during perfusion, then the flow rate out of the perfusion bioreactor should be s...

example 2

[0252] In a non-limiting example, the presently disclosed method comprises three separation steps, wherein each separation step is a chromatographic process, wherein each chromatographic step is a bind / elute process, such as Figure 7 The schematic process timing diagram is shown.

example 3

[0254] According to another non-limiting example, the presently disclosed method comprises three separation steps, wherein each separation step is a chromatographic process, wherein the first and second chromatographic steps are bind / elute processes, and the third chromatographic step is flow through the process, such as Figure 8 The schematic process timing diagram is shown.

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Abstract

The present disclosure is directed to method (100) for separating a biomolecule from a fluid (2), wherein the biomolecule is produced by biological cells in a perfusion bioreactor (3), the method comprising: forwarding (110) a product fluid comprising the biomolecule and biological cells from the perfusion bioreactor (3) during a bioreactor product output time period; loading (210) a first separation device (4) with the product fluid obtained in step (110) during a first separation loading time period, which constitutes a first part of a first separation cycle time period; pausing (130) the forwarding of the product fluid from the perfusion bioreactor during a bioreactor non-product output time period; and pausing (220) the loading of the first separation device (4) during a second part of the first separation cycle time period. Also disclosed are a computer-implemented method performed by a controller configured to control a separation of a biomolecule from a fluid (2), and further a controller, a computer program, and a computer program product.

Description

technical field [0001] The present disclosure relates to the field of separation of biomolecules, and is particularly directed to a method for isolating biomolecules from a fluid, more particularly wherein the biomolecules are produced by biological cells in a perfused bioreactor. Further disclosed is a computer-implemented method performed by a controller configured to control a method for isolating biomolecules from a fluid, and a controller operative to perform any of the steps of the disclosed method, a A computer program and a computer program product. Background technique [0002] Production of biomolecules in biological host cells by use of perfusion bioreactors is known in the art. The main advantages of the perfusion mode compared to batch / fed-batch are high cell numbers and high productivity in bioreactors of relatively small size. Features of a perfusion process for producing target biomolecules in cells include that culture medium is continuously supplied to th...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M1/00C07K1/36B01D15/18B01D15/42
CPCC12M47/10C07K1/36B01D15/1871B01D15/424B01D15/203C07K1/22C12M23/14C12M29/10C12M41/26
Inventor N·容内柳斯A·卡斯坦
Owner CYTIVA SWEDEN AB