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InDel molecular marker for identifying ball cracking resistance character of cabbage and application thereof

A molecular marker and cabbage technology, which is applied in the direction of recombinant DNA technology, microbial measurement/inspection, DNA/RNA fragments, etc., can solve the problems of being easily affected by environmental conditions, long cycle, economic loss, etc., and achieve the effect of improving breeding efficiency

Active Publication Date: 2022-02-01
SHANGHAI ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Cabbage cracking bulbs not only seriously affect the appearance quality and commerciality of leaf bulbs, but also easily cause insect pests and diseases, resulting in a significant reduction in the yield and storage and transportation resistance of cabbage, causing huge economic losses to production
The occurrence of cracking bulbs in cabbage is affected by many factors such as genetics, physiology and environment, but genetics is the main factor. Therefore, traditional agronomic prevention measures such as controlling fertilizer and water during the heading period are not effective. Only by cultivating cabbage varieties that are resistant to cracking bulbs can Fundamentally solve the problem of cabbage cracking
[0004] When conventional cross-breeding methods are used to select varieties resistant to cracking bulbs, breeders need to conduct field identification of cracking bulbs-resistant traits during the heading stage of cabbage, which takes a relatively long period and is easily affected by environmental conditions, time-consuming and low selection efficiency

Method used

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  • InDel molecular marker for identifying ball cracking resistance character of cabbage and application thereof
  • InDel molecular marker for identifying ball cracking resistance character of cabbage and application thereof
  • InDel molecular marker for identifying ball cracking resistance character of cabbage and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Example 1. Development of InDel molecular markers for auxiliary screening of cabbage blasting resistance

[0030] 1. Screening of InDel molecular markers for auxiliary screening of blastomere resistance in cabbage

[0031] According to the Bol016058 gene sequence of Brassica oleracea 02-12 reference genome in Phytozome (https: / / phytozome-next.jgi.doe.gov / ), design the full-length sequence amplification primer of this gene, described primer is:

[0032] Bol016058_1F: 5'-CCGAGTCCCAATTATTGTTGGTAAA-3';

[0033] Bol0160581R: 5'-GGACAAGGCTTCTTAGTCGGTGGTT-3';

[0034] Bol016058_2F: 5'-GGTTCCAGTGTACGAGCCACCT-3';

[0035]Bol016058_2R: 5'-ATCGCTATGTTTCTTGTAATGGTC-3';

[0036] Sangon Bioengineering (Shanghai) Co., Ltd. was commissioned to synthesize the above primers.

[0037] Genomic DNA of crack-resistant cabbage material "Laohongganlan" (old red cabbage) and easy-crack cabbage material "Shuanghuan" (double ring) were extracted by CTAB method.

[0038] Using the genomic DNA...

Embodiment 2

[0050] Example 2. Verification of the accuracy of the developed InDel molecular markers for screening cabbage cracking-resistant materials

[0051] In this example, the InDel molecular marker for the resistance to cracking bulbs of cabbage developed in Example 1 was used to identify the cracking bulbs-resistant traits of cabbage. The InDel molecular marker for the blasting-resistant trait of cabbage is a double-stranded DNA molecule shown in the 782-826 positions of SEQ ID No.1. The specific method is as follows:

[0052] 1 material

[0053] 21 cabbage inbred lines, as follows:

[0054] 16 servings of easy-to-crack cabbage: "Shuanghuan" (double ring), "011-81", "Helan2gunyuan" (Dutch 2 rolled round), "Dazhengfu" (big fight for father), "Niuxin" (beef heart), "Xiaoshanghai" ( Little Shanghai), "Eganlan0901" (Eganlan 0901), "3haojiangsujinyuan" (No. ), "Changzhengniuxin" (Long March Niuxin), "011-71", "011-83", "Zhengmu" (competing mother), "011-69".

[0055] 5 servings of ...

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Abstract

The invention discloses an InDel molecular marker for identifying the ball cracking resistance character of cabbage and application thereof. The invention specifically discloses an application of a cabbage ball cracking resistance character InDel molecular marker or a substance for detecting the cabbage ball cracking resistance character InDel molecular marker in identification or auxiliary identification of the cabbage ball cracking resistance character, and the cabbage ball cracking resistance character InDel molecular marker is a DNA molecule as shown in the 782-826th site of a nucleotide sequence SEQ ID No.1. The primer of the InDel molecular marker is designed, and a method for identifying or assisting in identifying the ball cracking resistance character of the cabbage is provided. The cabbage ball cracking resistance character InDel molecular marker is high in accuracy, good in stability and simple and feasible in detection operation, accelerates a cabbage selective breeding process, and can be widely applied to aspects such as cabbage germplasm resource analysis and molecular assisted genetic breeding.

Description

technical field [0001] The invention relates to the technical field of molecular markers, in particular to an InDel molecular marker for identifying the cracking-resistant character of cabbage and its application. Background technique [0002] Heading cabbage (Brassica oleracea L.var.capitata L.), referred to as cabbage, is a biennial vegetable crop of the genus Brassica in the family Cruciferae, and occupies a very important position in the annual supply of vegetables. [0003] Blast bulb is one of the main physiological obstacles common in cabbage cultivation. In the late stage of cabbage head formation, the outer leaves of the leaf ball are close to maturity and grow slowly. If the inner leaves continue to grow rapidly, the outer leaves will be split under the action of mechanical force. The most common is that the top of the leaf bulb is cracked, and sometimes the side is also cracked. In the lighter case, only the outer layers of the leaf bulb are cracked, and in sever...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6895C12Q1/6858C12N15/11
CPCC12Q1/6895C12Q1/6858C12Q2600/13C12Q2600/156C12Q2531/113C12Q2565/125
Inventor 朱晓炜薄天岳陈锦秀邰翔王敏
Owner SHANGHAI ACAD OF AGRI SCI
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