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Culture method and application of selenium and sulforaphane enriched broccoli bud seedlings

A technology for broccoli sprouts and a cultivation method, which is applied to the cultivation of broccoli sprouts enriched with selenium and sulforaphane, and the field of yogurt, can solve the problems of troublesome operation, high cost, limited overall concentration of selenium, etc. Effects of increased sulfur content, enhanced tolerance, and high enrichment

Active Publication Date: 2022-02-08
NORTHWEST UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] An existing method for enriching broccoli sprouts with glucoraphanin and selenoamino acids is to apply exogenous selenium and put the sprouts under high temperature stress once a day at 35-60°C for 15-25 minutes each time , this method needs to continuously provide exogenous energy in the process of sprout germination, the operation is cumbersome and the cost is high, and the highest concentration of exogenous selenium applied is only 1mM, so that the overall enrichment concentration of selenium in the sprout is limited, and, Due to the competitive inhibition effect between selenium and sulfur, the synthesis of sulforaphane will be affected after the application of exogenous selenium in the cultivation of broccoli sprouts

Method used

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  • Culture method and application of selenium and sulforaphane enriched broccoli bud seedlings
  • Culture method and application of selenium and sulforaphane enriched broccoli bud seedlings
  • Culture method and application of selenium and sulforaphane enriched broccoli bud seedlings

Examples

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preparation example Construction

[0061] In some preferred modes, the broccoli sprout powder is prepared by the following steps:

[0062] 1) Sprouts blanching: take broccoli sprouts, put them in a water bath at 55°C for 15 minutes, and cool them down rapidly;

[0063] 2) Enzymolysis: mix the blanched broccoli sprouts with 4-5 times (w / v) water, add 0.1-0.2% ascorbic acid, adjust the pH to 4-4.5 with citric acid, and then place it at 35- Enzymolysis in a constant temperature shaker at 40°C for 5-6 hours;

[0064] 3) Freeze-drying: After the enzymolysis is completed, the enzymolysis solution is centrifuged at a high speed at 10,000 rpm, rotated at 45-55°C, and taken out after being concentrated to a quarter of its volume;

[0065] Add 2-3% (w / v) cyclodextrin and 5-8% (w / v) maltodextrin to the concentrated solution to dissolve, put it in the refrigerator and freeze it; Freeze-dry in a freeze dryer to obtain the broccoli sprout powder.

[0066] The broccoli sprout powder of the present application is rich in su...

Embodiment 1

[0075] (1) Seed screening and low-temperature plasma pretreatment: select broccoli seeds that are produced in the current year, without damage and mildew, without impurities, and with full particles, and perform plasma pretreatment. The pretreatment voltage is 20KV and the current is 0.6A. , the time is 2min, and the processing environment temperature is room temperature;

[0076] (2) Seed disinfection and seed soaking: soak the pretreated seeds with 0.5% sodium hypochlorite for 5 minutes, then switch to soaking in water for 6 hours, and then rinse with water for 3 times;

[0077] (3) Germination cultivation: the broccoli seeds after cleaning are placed in the culture dish, and the culture dish is covered with water-absorbing gauze, and the culture dish is placed in a temperature of 25° C., and cultivated in an incubator with a humidity of 70%; Spray a selenium-containing nutrient solution containing 1mM sodium selenate, 1mM zinc sulfate and 50μM magnesium sulfate once every 6...

Embodiment 2

[0079] Broccoli sprouts were cultivated according to the cultivation method of Example 1, the difference being that the pretreatment voltage was 25KV, the current was 0.8A, and the time was 3min.

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Abstract

The invention discloses a culture method and application of selenium and sulforaphane-enriched broccoli bud seedlings. The culture method comprises the following steps: broccoli seeds are pretreated by adopting low-temperature plasma; and the pretreated seeds are soaked and then subjected to germination culture, a selenium-containing nutrient solution is sprayed in the culture process, and the broccoli bud seedlings rich in selenium and sulforaphane are obtained. The application accidentally finds that after the broccoli seeds are pretreated by adopting the low-temperature plasma, the content of sulforaphane in the obtained broccoli bud seedlings is remarkably increased, the tolerance of the broccoli bud seedlings to selenium is enhanced, and the highest applicable exogenous selenium concentration can reach 3mM; and the growth of the bud seedlings and the accumulation of the sulforaphane are not obviously inhibited, and meanwhile, the high enrichment of the selenium and the sulforaphane in the broccoli bud seedlings is realized.

Description

[0001] This application claims the priority of the Chinese invention patent application with the application date of September 2, 2021 and the application number of 2021110663862. technical field [0002] This application relates to the field of food technology, in particular to a broccoli sprout cultivation method enriched in selenium and sulforaphane and its application, more specifically, the application relates to a broccoli sprout cultivation enriched in selenium and sulforaphane method, a kind of broccoli sprouts, a kind of broccoli sprout powder and a kind of yogurt. Background technique [0003] Sulforaphane is a plant-derived chemical substance with strong detoxification, anti-cancer and anti-cancer activities. Broccoli sprouts are rich in sulforaphane precursor glucoraphanin, and its glucoraphanin content is 20 to 100 times that of mature broccoli , glucoraphanin can be synthesized into sulforaphane after being enzymatically hydrolyzed by endogenous myrosinase in p...

Claims

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Application Information

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IPC IPC(8): A01G31/00A01C1/00A23C9/133A23L19/00
CPCA01G31/00A01C1/00A23C9/133A23L19/01
Inventor 吕新刚高诗薇朱禹蒙范代娣
Owner NORTHWEST UNIV
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