Application of migration type abietane diterpenoid compound in preparation of medicine for kidney protection
A compound and precursor compound technology, applied in the field of biomedicine, can solve the problem of lack of effective treatment of kidney injury, etc., and achieve the effect of improving serum kidney index, good protective effect, and good serum kidney index
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Embodiment 1
[0045] Example 1: Preparation and structure identification of SIPI-10
[0046] 1.1 Preparation of SIPI-10
[0047] 1) Extraction: take the Chinese orchid medicinal material and add 70% ethanol to reflux extraction, the amount of 70% ethanol is 10 (v / m) times of the medicinal material, extract twice, each time for 1 hour, filter the extracts and combine, dry, Dexiangqinglan dry extract. The extract was suspended in 2 times the amount of water, extracted sequentially with petroleum ether, dichloromethane, ethyl acetate and n-butanol to obtain ethyl acetate extract.
[0048] 2) Column chromatography: The obtained ethyl acetate extract was subjected to silica gel column chromatography, and petroleum ether: acetone solution (100:1 → 50:1 → 20:1 → 10:1 → 5:1 → 2 :1→1:1) gradient elution, the amount of mixed solvent used for each gradient is 3 times the column volume (v / v) (that is, the volume of the solvent used is 3 times the volume of the silica gel column), and the volume of ea...
Embodiment 2
[0058] Example 2: Detection of serum biochemical indicators, observation of renal tissue necrosis and survival rate of mice, and analysis of the effect of SIPI-10 on renal ischemia-reperfusion mice.
[0059] The bilateral renal pedicle clipping method is a commonly used modeling method at home and abroad. By suddenly and temporarily blocking the blood supply to the kidneys, the mitochondrial dysfunction of the cells and the energy production decline; when the blood is restored to reperfusion, a large amount of active oxygen is released to cause lipid peroxidation of the membrane, causing rapid damage to the kidney tissue.
[0060] 2.1 Experimental method
[0061] Fifty-six SPF-grade ICR healthy male mice weighing 25±2 g were provided by Shanghai Slack Experimental Animal Co., Ltd. The room temperature is 22-25°C, the humidity is 60%-70%, and the day and night cycle is 12h, free to eat. Animals were randomly divided into 7 groups, 8 in each group, respectively sham operation ...
Embodiment 3
[0082] Example 3: Detection of serum biochemical indicators and observation of renal pathological tissue, analysis of the protective effect of SIPI-10 on LPS-induced acute kidney injury
[0083] Severe infection and septic shock are one of the most common causes of acute kidney injury. Epidemiological studies have shown that systemic inflammatory infection caused by sepsis is the most important cause of clinical infectious AKI. Endotoxin is a structural component in the cell wall of Gram-negative bacteria, which can activate the release of various inflammatory factors in the body, and then cause the body's immune verification response.
[0084] 3.1 Experimental method
[0085] Twenty healthy male ICR mice of SPF grade, weighing 25±2 g, were provided by Shanghai Slack Experimental Animal Co., Ltd. The room temperature is 22-25°C, the humidity is 60%-70%, and the day and night cycle is 12h, free to eat. The animals were randomly divided into 4 groups, 5 animals in each group, ...
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