Carrier protein based on subject-object self-assembly and application thereof

A host-guest self-assembly, carrier protein technology, applied in the field of vaccine development, can solve the problems of vaccine interference, carbohydrate antigen immunosuppression, etc., and achieve the effects of promoting uptake, reducing immune response, and shortening immune response time

Pending Publication Date: 2022-02-15
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In addition, only a few carrier proteins are clinically approved for use in the vaccine industry, and the use of the s

Method used

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  • Carrier protein based on subject-object self-assembly and application thereof
  • Carrier protein based on subject-object self-assembly and application thereof
  • Carrier protein based on subject-object self-assembly and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0029] Example 1

[0030] One of the main objects of the present invention is electrically used in the carrier protein, at least one diamondane structure, which can be used for assembly with cyclodextrin, and the syndrome structure is assembled by the main passenger body. Forming a complex with Beta-cyclodextrin and its derivatives;

[0031] The carrier protein is selected from the bull serum albumin or human serum albumin, or two or more fusion proteins.

[0032] The BETA-cyclodextrin and its derivatives, the hydroxyl group of Beta-cyclodextrin is modified.

[0033] The conjugate based on the primary passenger self-assembled carrier protein is the conjugate obtained by the main guest self-assembled carrier protein to the target antigen, wherein the target antigen is a tumor antigen.

[0034] The target antigen is a tumor-related sugar antigen.

[0035] The tumor-associated sugar antigen is a derivative of TF, TN, STN, Globo H, GM2, GM3, GD2, GD3, MUC1, and MUC1.

[0036] The pres...

Example Embodiment

[0040] Example 2

[0041] Example 2 The difference from Embodiment 1 is that the carrier protein is selected from the group consisting of oocytoproteins. The target antigen is a bacterial antigen.

Example Embodiment

[0042] Example 3

[0043] Example 3 Difference with Example 1 is that the carrier protein is selected from the group consisting of oval protein, diphtheria toxin, white-throatotoxin non-toxic mutant CRM197, tethaal toxin, keylet blood blue protein, bacterial expression protein A fusion protein formed in any one or more or more. The target antigen is a viral antigen.

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Abstract

The invention discloses a carrier protein based on subject-object self-assembly and application of the carrier protein in vaccines. At least one adamantane structure is chemically coupled and modified on the carrier protein; the carrier protein is a fusion protein formed by one or more than two of bovine serum albumin, human serum albumin, ovalbumin, diphtheria toxoid, a non-toxic mutant of the diphtheria toxoid, tetanus toxoid, keyhole limpet hemocyanin and bacterial expression protein; and the adamantane modified carrier protein, beta-cyclodextrin and a derivative (beta-cyclodextrin-rhamnose) of beta-cyclodextrin form a subject-object self-assembly structure. The subject-object self-assembled carrier protein can promote phagocytosis, treatment and presentation of antigen presenting cells, so that the immunogenicity of vaccine molecules is effectively improved, and the antigen-related immune response is improved. According to the present invention, the immunoreaction on the conjugated exogenous antigen is enhanced, the immunoreaction time can be shortened, and the immunization frequency can be reduced.

Description

Technical field [0001] The present invention relates to the field of vaccine development, and in particular, to the main object-based carrier protein and its application thereof. Background technique [0002] In the process of developing tumor-related sugar antigen vaccines, people often observe that the immune response to the carrier protein is very strong, and there is no treatment to the sugar antigen. A large number of peptide surfaces present in the carrier protein may be limited with a limited number of adjuvant T cells and B cells that have a concentrated amount of sugar resistance, resulting in undesirable activation or differentiation signals and inhibiting the immune response to sugar antigens. In addition, only a few carrier proteins are approved for the vaccine industry, and the same carrier is used in different vaccines, which may cause vaccine interference, which in turn leads to immunosuppression against the sugar antigen. [0003] Therefore, a new carrier is requi...

Claims

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Application Information

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IPC IPC(8): A61K47/69A61K39/385A61K39/00A61P35/00C07K14/765
CPCA61K47/6951A61K39/385A61K39/0011C07K14/765A61P35/00A61K2039/6081A61K2039/6037
Inventor 周志昉吴志猛林汉李艳春周坤俞杭艳
Owner JIANGNAN UNIV
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