Strip color developing type test strip for detecting oxalic acid in urine as well as preparation method, use method and application of strip color developing type test strip

Abstract

The invention discloses a strip color developing type test strip for rapidly detecting the concentration of oxalic acid in urine as well as a preparation method, a use method and application of the strip color developing type test strip. The test strip is formed by overlapping four parts, namely a sample pad, a combination pad, a reaction film and an absorption pad. The method comprises the following steps: loading oxalate oxidase and horse radish peroxidase on the combination pad of the test strip; and a plurality of lines are uniformly scribed on the reaction film at intervals by using a color developing agent. The detection limit of the test strip is lower than 0.3 mM, and the detection range covers the concentration range of oxalic acid in urine from normal people to patients with hyperoxaluria. The method does not need image acquisition and processing equipment depended on by a traditional colorimetric method, realizes'equipment-free 'detection of oxalic acid, and has the advantages of simplicity and convenience in operation, low cost and capability of instantly detecting on site; the test strip can be prepared on a large scale, is widely applicable to household monitoring and bedside detection, and has a good application prospect.

Description

technical field [0001] The invention belongs to the technical field of immediate testing, and in particular relates to a strip color-developing test paper strip and its preparation, and a method for semi-quantitatively detecting oxalic acid in urine and its application. Background technique [0002] Kidney stones are one of the most common diseases in urology. The prevalence rate of the global population is as high as 5%-10%, and the incidence and prevalence are increasing worldwide, and there is a high possibility of recurrence within 5-10 years sex. Among various types of kidney stones, calcium oxalate stones are the stones with the highest incidence rate in the urinary system. It has been clinically proven that the excretion of oxalate in urine plays a key role in the formation of calcium oxalate stones, and hyperoxaluria is considered to be a clinical indicator highly correlated with calcium oxalate stones. At present, it is lagging behind in disease prevention and tre...

AI Technical Summary

Problems solved by technology

The above detection methods have high sensitivity and specificity, but most rely on expensive and complicated equipment, a large number of samples, toxic and expensive reagents and professional technicians, and are not suitable for point-of-care testing (POCT)

However, relatively new detection methods such as colorimetric sensors based on enzymatic methods greatly reduce detection requirements and costs, but still require image acquisition and analysis equipment to obtain results

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