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Cell therapeutic agent for treating premature ovarian failure by using umbilical cord blood concentrated cells

A technology of umbilical cord blood and cell preparations, which is applied in the fields of biotechnology and biomedicine, and can solve problems such as complex operations, poor repeatability of results, and easy contamination

Pending Publication Date: 2022-03-15
BOYALIFE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] However, umbilical cord blood is traditionally separated by density gradient centrifugation by manual operation, which has very difficult problems such as complicated operation, long time-consuming, easy contamination, and poor reproducibility of results.

Method used

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  • Cell therapeutic agent for treating premature ovarian failure by using umbilical cord blood concentrated cells
  • Cell therapeutic agent for treating premature ovarian failure by using umbilical cord blood concentrated cells
  • Cell therapeutic agent for treating premature ovarian failure by using umbilical cord blood concentrated cells

Examples

Experimental program
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Effect test

Embodiment 1

[0108] Example 1: Rapid separation and preparation of concentrated umbilical cord blood cell preparation (UCBC)

[0109] (1) Provide biological samples of umbilical cord blood (20-350ml samples can be processed), put them in a sterile bag containing anticoagulant for use, and take another 1ml sample for testing;

[0110] (2) Remove the protective cap on the input tube of the automatic cell separation system, connect the syringe to the Luer lock connector of the input tube, pass through the thrombus filter at a slow and steady speed, and transfer the anticoagulated biological sample to a disposable In the sterile separation cup, shake the mixed sample along the horizontal axis; the automatic cell separation system is a closed PXP separation system, which consists of four parts: a) disposable sterile separation cup, b) control module, c) Separate base for data transmission, d) DataTrak software processing system;

[0111] (3) Put the disposable separation cup into the control...

Embodiment 1a： reference Embodiment 1 preparation preparation ，，5 to 5 preparation 。 Embodiment 1b： reference Embodiment 1 preparation preparation ，，5 to 5 preparation 。 Embodiment 1c

[0124] Example 1a: Refer to Example 1 to prepare umbilical cord blood concentrated cell preparations, the difference is that no histidine is added to the anticoagulant, and 5 biological samples are processed to obtain 5 umbilical cord blood concentrated cell preparations. Example 1b: Refer to Example 1 to prepare concentrated cord blood cell preparations. The difference is that phosphatidylcholine is not added to the anticoagulant, and 5 biological samples are processed to obtain 5 cord blood concentrated cell preparations. Example 1c: Refer to Example 1 to prepare the cord blood concentrated cell preparation, the difference is that neither histidine nor phosphatidylcholine is added to the anticoagulant, and 5 biological samples are processed to obtain 5 cord blood concentrates cell preparations. The blood plasma layer obtained in the operation of embodiment 1, embodiment 1a, embodiment 1b, embodiment 1c is diluted 1 times with double distilled water, measures absorbance at 54...

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Abstract

The invention relates to a cell therapeutic agent for treating premature ovarian failure by using umbilical cord blood concentrated cells. On one hand, the method for preparing the umbilical cord blood concentrated cell preparation comprises the following steps: providing a biological sample umbilical cord blood, and putting the biological sample umbilical cord blood into a sterile bag containing an anticoagulant for later use; a protective cap on an input tube of the automatic cell separation system is taken down, an injector is connected to a Luer locking connector of the input tube and passes through a thrombus filter at a slow and stable speed, an anticoagulant biological sample is transferred into a disposable sterile separation cup, and the mixed sample is shaken along a horizontal shaft; and putting the disposable separation cup into a control module, and centrifuging in a programmable centrifugal machine to obtain the umbilical cord blood concentrated cell preparation. The invention also relates to the umbilical cord blood concentrated cell preparation prepared by the method, and application of the umbilical cord blood concentrated cell preparation in preparation of a cell therapeutic agent for treating premature ovarian failure. The cell preparation disclosed by the invention shows an excellent effect.

Description

technical field [0001] The invention belongs to the fields of biotechnology and biomedicine, and relates to a method for treating premature ovarian failure (Premature ovarian failure, POF) by using a cell therapy agent. Specifically, the present invention relates to a method for rapidly separating and preparing cord blood concentrated cells (cord blood concentrate) from umbilical cord blood to treat premature ovarian insufficiency and premature ovarian failure using the cord blood concentrated cells. Using the method of the present invention can effectively improve the efficiency of isolating umbilical cord blood concentrated cells from umbilical cord blood, and provide a safe, efficient and simple preparation of umbilical cord blood concentrated cells for treating premature ovarian failure and ovarian insufficiency such as premature ovarian insufficiency Methods. by using The automatic cell rapid processing system achieves the object of the present invention. Background ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K35/51A61K47/12A61P15/08C12N5/078
CPCA61K35/51A61K47/12A61P15/08C12N5/0634C12N2509/10
Inventor 肖海蓉李容徐勇
Owner BOYALIFE
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