Automatic focusing method for bone marrow smear

Abstract

The invention relates to an automatic focusing method for a bone marrow smear, which comprises the following steps: (1) a mechanical arm is moved up and down to drive the bone marrow smear to shoot a target cell under a dark field light path, the shooting position of the mechanical arm is Pos1, the corresponding logic value of the mechanical arm is X1, and then the mechanical arm is moved up and down for 9 times to obtain 19 logic values in total, taking a difference coordinate between the 19 logic values and X1 as X; (2) each X value corresponds to a cell hologram of the bone marrow smear shot by a camera under a dark field light path (digital holographic microscope) and a hologram of a blank background, opencv (Matlab) is utilized to carry out fast Fourier transform on the holograms, the shot holograms are converted into a frequency domain from a space domain, and a spectrogram is obtained; (3) intercepting + 1-level or-1-level frequency spectrum in the spectrogram, filling zero in the frequency spectrum area which is not intercepted, respectively carrying out inverse Fourier operation and phase taking, and subtracting the phase of the blank background hologram from the phase of the cell hologram; according to the invention, an automatic focusing algorithm is utilized, the focusing time for bone marrow smear shooting is shortened, and the bone marrow smear shooting efficiency is greatly improved.

Description

technical field [0001] The invention belongs to the field of artificial intelligence medical diagnosis, and in particular relates to an automatic focusing method for bone marrow smears. Background technique [0002] At present, the scanning equipment for bone marrow smear generally places the bone marrow smear on the stage, the microscope lens is installed on the frame, and the stage moves up and down to achieve the focus on the bone marrow smear. The main technical difficulties are as follows: microscope The depth of field of the 100-fold lens is relatively small, and when making bone marrow smears, the uneven thickness of the smears, the difference in cell size, and the existence of impurities on the smears often require high precision in hardware equipment and algorithms. . [0003] At present, the focusing methods of smears are mainly as follows: 1. The focusing is completed through rough adjustment first, and then fine adjustment, and the processing efficiency is low. ...

AI Technical Summary

Problems solved by technology

[0003] At present, the focus methods of smears are mainly as follows: 1. The focus is completed by rough adjustment first, and then fine adjustment, and the processing efficiency is low.

2. With the help of measuring tools, such as laser range finders, first measure the relative height of the target, and then move the stage to the focal point according to the measurement data. This method has high requirements for the accuracy of the measuring tool, the flatness and cleanliness of the smear, and cannot be guaranteed The main observation object in the actual focus shooting image must be clear, there is randomness

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