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Spodoptera frugiperda beauveria bassiana Bbsfa202007 strain and application thereof

A technology of bbsfa202007 and Beauveria bassiana is applied in the field of microorganisms to achieve the effects of avoiding drug resistance and environmental pollution, simple cultivation methods, and large development and application potential.

Active Publication Date: 2022-04-12
YUNNAN AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] For the above-mentioned defects of the prior art, the object of the present invention is to provide a Beauveria bassiana Bbsfa202007 and its application, aiming to overcome the deficiency that the existing Beauveria bassiana can parasitize the pupa of Spodoptera frugiperda. Technical means to prevent and control the pupae of Spodoptera frugiperda, to avoid or reduce the problem of drug resistance caused by unreasonable use of chemical pesticides

Method used

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  • Spodoptera frugiperda beauveria bassiana Bbsfa202007 strain and application thereof
  • Spodoptera frugiperda beauveria bassiana Bbsfa202007 strain and application thereof
  • Spodoptera frugiperda beauveria bassiana Bbsfa202007 strain and application thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Isolation and identification of pathogenic bacteria

[0023] 1.1 Materials and methods

[0024] 1.1.1 Materials

[0025] Diseased adults of Spodoptera frugiperda were collected from Dali, Yunnan Province after infestation by an entomogenic fungus.

[0026] Sabouraud medium (SDAY): 1% peptone + 1% yeast powder + 4% glucose + 1.5-2% agar powder + 1000mL water.

[0027] Sterile operating conditions: All utensils and utensils are sterilized in a high-temperature autoclave (121°C, 30min), and inoculation and other operations are performed in an ultra-clean workbench.

[0028] Culture conditions: Culture in a 28°C light (12L: 12D) incubator. After the colonies are formed, transfer to the SDAY slant of the test tube, cultivate for 2-3 days, and transfer to a 4°C refrigerator for storage.

[0029] 1.1.2 Isolation and purification of pathogenic bacteria

[0030] Isolation: Isolate the pathogenic bacteria from the carcass of the sick Spodoptera frugiperda adults. Take the di...

Embodiment 2

[0040] Biological Characteristics of Purified Strains of Beauveria bassiana

[0041] 2.1 Materials and methods

[0042] 2.1.1 Tested strains

[0043] Select a plate with vigorous growth and uniform growth after purification as the test strain. The hyphae were inoculated on SDAY again, and cultivated in a constant temperature light (12L:12D) incubator at 25°C.

[0044] 2.1.2 Determination of colony growth rate and sporulation

[0045] Inoculate the isolated and purified Beauveria bassiana strain Bbsfa202007 on SDAY and PDA medium (potato 200g, glucose 20g, agar 20g, distilled water 1L), at (25±1)°C, relative humidity 70%, L:D =14h: cultivated in a 10h light incubator (Hengli (China) Laboratory Equipment Technology Co., Ltd., model RG-300). On the 3rd, 7th and 14th day of inoculation, the diameter of the colony was measured and the morphological characteristics of the colony were observed. The microstructure of the sporulation structure and conidia morphology of the strain ...

Embodiment 3

[0051] Indoor Toxicity Determination of Beauveria bassiana Bbsfa202007 to Spodoptera frugiperda pupae

[0052] 3.1. Materials and methods

[0053] 3.1.1 Source of tested insects

[0054] The pupae of Spodoptera frugiperda (J.E.Smith, 1797) were reared with sugarcane in our laboratory at a temperature of (25±1)°C, a humidity of (80±5)%, and a photoperiod of 12L:12D in an incubator. Healthy, two-day-old pupae of the same size were obtained as test insects.

[0055] 3.1.2 Preparation of spore suspension

[0056] Get the purified Beauveria bassiana with good growth and wash the conidia with sterile water and filter to make 10 8 Spores / mL conidia suspension, use a sterile capillary dropper to take the conidia suspension and drop it on a blood cell counting plate, count the spores under a microscope and record the data, use sterile water containing 0.5% Tween 80 serially diluted to 10 8 、10 7 、10 6 、10 5 and 10 4 The spore suspensions with five concentration gradients and s...

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Abstract

The invention discloses a strain of spodoptera frugiperda beauveria bassiana Bbsfa202007 and application thereof, the strain of the spodoptera frugiperda beauveria bassiana Bbsfa202007 is Beauveria bassiana, the preservation name of the strain is Beauveria bassiana, and the strain of the spodoptera frugiperda beauveria bassiana Bbsfa202007 is a strain of the spodoptera frugiperda beauveria bassiana Bbsfa202007 and application of the strain of the spodoptera frugiperda beauveria bassiana Bbsfa202007. The strain is preserved in China General Microbiological Culture Collection Center (CGMCC), and the preservation address is No.3, Yard 1, Beichen West Road, Chaoyang District, Beijing; the preservation date is May 31, 2021; the preservation number is CGMCC (China General Microbiological Culture Collection Center) The strain is separated from the adult body of the spodoptera frugiperda for the first time, and is relatively simple to culture, rapid in growth and large in sporulation quantity. The compound has strong pathogenicity to spodoptera frugiperda pupa, is environment-friendly and pollution-free, is not easy to generate drug resistance, and can be widely used for preventing and treating spodoptera frugiperda pupa.

Description

technical field [0001] The invention relates to the technical field of microorganisms, in particular to a Beauveria bassiana Bbsfa202007 strain of Spodoptera frugiperda and its application. Background technique [0002] Spodoptera frugiperda (J.E.Smith, 1797), also known as army worm and fall armyworm, belongs to Lepidoptera Lepidoptera family Noctuidae Spodoptera, which is native to tropical and subtropical regions of America. It has a wide appetite, large food intake, and strong reproductive and migratory abilities. It has developed into a global pest. On January 11, 2019, it was first discovered in Jiangcheng County, Pu'er City, Yunnan Province that the larvae of Spodoptera frugiperda harmed winter corn, and then in 26 provinces across the country hazards were monitored. Spodoptera frugiperda mainly feeds on corn, sugarcane, wheat and other gramineous crops. It is especially harmful to corn and has become an important pest affecting corn production. The insect is an imp...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14A01N63/30A01P7/04C12R1/645
Inventor 陈斌杜广祖庞继鑫温绍海薛锐付玉飞张栩孙淦琳
Owner YUNNAN AGRICULTURAL UNIVERSITY
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