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Anaerobic river sediment degrading strain and application thereof

A technology of river sediment and strains, applied in the field of environmental microorganisms, can solve problems such as human health and ecological environment risks, and achieve the effects of reduction and harmlessness, high application value, and convenient operation

Active Publication Date: 2022-04-15
FOSHAN YUHUANG ECOLOGICAL ENVIRONMENT TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In addition, the organisms in the sediment can easily ingest the pollutants in the sediment. After entering the food chain, the pollutants will be enriched in the organisms, posing serious risks to human health and the ecological environment.

Method used

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  • Anaerobic river sediment degrading strain and application thereof
  • Anaerobic river sediment degrading strain and application thereof
  • Anaerobic river sediment degrading strain and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Example 1: Isolation, screening and performance determination of anaerobic river sediment-degrading bacteria

[0031] The media and components used are as follows:

[0032] Liquid medium: glucose 5 g / L, tryptone 3 g / L, beef powder 2 g / L, NaCl 4 g / L, K 2 HPO 4 0.75 g / L, KH 2 PO 4 0.75 g / L, FeSO 4 ·7H 2 O 0.05 g / L, MgSO 4 ·7H 2 O 0.3 g / L, (NH 4 ) 2 SO 4 3 g / L, cysteine ​​0.25 g / L, natural pH.

[0033] Solid medium: glucose 5 g / L, tryptone 3 g / L, beef powder 2 g / L, NaCl 4 g / L, K 2 HPO 4 0.75 g / L, KH 2 PO 4 0.75 g / L, FeSO 4 ·7H 2 O 0.05 g / L, MgSO 4 ·7H 2 O 0.3 g / L, (NH 4 ) 2 SO 4 3 g / L, cysteine ​​0.25 g / L, 15 g / L agar powder, natural pH.

[0034] River sediment liquid medium: river sediment (the total solid TS content is 11%, and the VS content is 7.8% of TS, both are mass fractions). Add water to adjust the sediment TS content to 5%, and the pH value to 7.0.

[0035] River sediment solid medium: river sediment (TS content is 11%, VS content is 7...

Embodiment 2

[0042] Example 2: Biological Identification of Anaerobic River Sediment Degradation Strain YE6

[0043] Genomic DNA of strain YE6 was extracted and used as a template to amplify strain 16S rDNA with a pair of universal primers (27F, 1492R). The upstream primer is 27F (5'-AGAGTTTGATCCTGGCTCA-3'), and the downstream primer is 1492R (5'-GGTTACCTTGTTACGACTT-3').

[0044] The PCR reaction system (20 μL) is as follows: template DNA 0.5 μL, PCR Taqmix 10 μL, upstream and downstream primers 0.6 μL each, add ddH 2 O to 20 μL of reaction system.

[0045] PCR program: pre-denaturation at 94°C for 5 min, denaturation at 94°C for 30 s, annealing at 55°C for 30 s, extension at 72°C for 1 min and 30 s, and a total of 30 cycles of the above, extension at 72°C for 10 min, and finally storage at 4°C.

[0046] The PCR product was purified and sequenced by Shanghai Jieli Biotechnology Co., Ltd., and the 16S rDNA sequence of the strain was obtained as shown in SEQ ID NO.1. The 16S rDNA sequence...

Embodiment 3

[0048] Embodiment 3: Preparation of liquid microbial agent of anaerobic river channel sediment degradation strain YE6

[0049] The YE6 pure strain was inoculated in 10 mL of liquid medium, cultured anaerobically at 37 °C for 24 h, and then inserted into the liquid medium of the next stage of expanded culture with an inoculum volume of 5% of the volume of the liquid medium. The liquid microbial agent of the strain can be obtained by carrying out multi-stage anaerobic expansion culture under the same conditions.

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Abstract

The invention discloses an anaerobic river sediment degradation strain, namely bacillus haynesii YE6 and application of the bacillus haynesii YE6 in anaerobic degradation of river sediment, and relates to the technical field of environmental microorganisms. The strain bacillus haynesii YE6 disclosed by the invention is preserved in the China Center for Type Culture Collection, the preservation number of the strain is CCTCC NO: M 20211674, and the preservation date is December 24, 2021. The bacillus haynesii YE6 can effectively improve the degradation rate of organic matters in the river sediment in an anaerobic environment, can be used for reduction and harmless treatment of the river sediment, and has a relatively high application value.

Description

technical field [0001] The invention belongs to the technical field of environmental microbes, and in particular relates to an anaerobic river channel sediment-degrading strain and application thereof. Background technique [0002] With the rapid development of the economy and the rapid expansion of the population, a large amount of pollutants are discharged into the river, exceeding the self-purification capacity of the river, causing serious deterioration of the river and surrounding environment, and negatively affecting people's lives and urban development. Many toxic and harmful inorganic substances and persistent organic substances remain in the river sediment for a long time and accumulate in large quantities, making them complex in composition and difficult to degrade. When environmental factors change, organic pollutants such as nitrogen and phosphorus in the sediment will seep into the groundwater or be released into the overlying water. In addition, the organisms ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C02F11/04C12R1/07C02F101/30
CPCC12N1/20C02F11/04C02F2101/30C02F2103/007C02F2303/06
Inventor 邱建贺翁雯周国彪梁锡伟
Owner FOSHAN YUHUANG ECOLOGICAL ENVIRONMENT TECH
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