Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Marker and probe composition for screening liver cancer and application of marker and probe composition

A technology of markers and compositions, applied in the fields of application, combinatorial chemistry, organic compound library, etc., can solve the problem of reducing the 5-year total mortality rate of liver cancer, achieve high sensitivity and accuracy, and reduce the effect of harm

Pending Publication Date: 2022-04-19
BIOCHAIN BEIJING SCI & TECH
View PDF8 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Existing diagnosis and treatment strategies and measures are very limited in reducing the 5-year total mortality rate of liver cancer, so it is extremely urgent to explore new screening, diagnosis and treatment strategies for liver cancer

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Marker and probe composition for screening liver cancer and application of marker and probe composition
  • Marker and probe composition for screening liver cancer and application of marker and probe composition
  • Marker and probe composition for screening liver cancer and application of marker and probe composition

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0146] The preparation of the chip is mainly based on a glass sheet or a silicon sheet as a carrier, and oligonucleotide fragments or cDNAs are arranged on the carrier in sequence as probes by means of in situ synthesis and micro-array.

[0147] The chip of the present invention is based on the signal detection of DNA sequence hybridization after bisulfite treatment. Bisulfite treatment is to change unmethylated cytosine into uracil, while methylated cytosine remains unchanged. Change, then convert uracil to thymine, and finally carry out chip hybridization; finally judge the type of base added according to the fluorescent color, and then determine whether the site is methylated.

[0148] The present invention provides a method for liver cancer screening, which comprises:

[0149] detect the methylation level of the marker, and

[0150] The risk of the subject suffering from liver cancer is judged based on the methylation level, and the marker is selected from one of the foll...

Embodiment 1

[0153] Example 1 Screening markers

[0154] 1) Sample collection: Download the 450k methylation microarray cancer tissue data in TCGA, involving a total of 7,769 cancer tissue samples of 26 types of tumors, including adrenocortical carcinoma (80), bladder urothelial carcinoma (409), acute myeloid Leukemia (140), brain low-grade glioma (654), breast cancer (740), cervical cancer (286), liver cancer (348), esophageal cancer (183), uveal melanoma (80), head and neck squamous cell Carcinoma (527), kidney cancer (660), liver cancer (377), lung adenocarcinoma (425), lung squamous cell carcinoma (372), diffuse large B-cell lymphoma (29), ovarian serous cystadenocarcinoma (10) , pancreatic cancer (184), mesothelioma (116), prostate cancer (488), skin melanoma (104), sarcoma (117), gastric cancer (397), testicular cancer (134), thymus cancer (94), thyroid cancer cancer (506), endometrial cancer (309). For the healthy population, Boercheng collected the plasma of 38 healthy individual...

Embodiment 2

[0319] Based on 15 clinically diagnosed liver cancer samples collected from the Beijing area and 15 healthy human samples collected from the Beijing area, the 9 methylated biomarkers were calculated and screened using the methylation database construction method described in Example 1 According to the methylation level of the 9 methylation biomarkers in the liver cancer sample and normal human sample data sets, the threshold value (hereinafter referred to as the site or marker) and the AUC value for independent distinction are calculated in the table 18;

[0320] The calculation method of the methylation level threshold is: according to the data set (including the type and methylation level of each sample), the ROC curve is drawn, and the confusion matrix corresponding to the optimal threshold point on the ROC curve will be our calculated sensitivity ( sensitivity), specificity, and accuracy. Normally, we will use the Youden index to make selections. Youden index, also known...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a marker and a probe composition for screening liver cancer and application of the marker and the probe composition. The marker is selected from any one of nine markers. According to the present invention, with the application of the marker, the methylation state of the gene can be sensitively and specifically detected so as to be used for the detection of the peripheral blood free DNA, and the composition is used for the screening of the asymptomatic population in the non-invasive manner so as to reduce the harm caused by the invasive detection; the composition has higher sensitivity and accuracy, and can realize real-time monitoring.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a marker for liver cancer screening, a probe composition and applications thereof. Background technique [0002] my country is a big country with liver cancer. About half of the world's liver cancer occurs in China. The five-year overall survival rate of liver cancer is only 14.1%. The main reason for the low long-term survival rate of liver cancer is that the screening of high-risk groups of liver cancer is not popularized, and the early diagnosis rate is low, resulting in 70% to 80% of patients are already in the middle and late stage at the time of diagnosis. If early detection and early diagnosis can be implemented Radical methods such as liver resection and liver transplantation can significantly improve the prognosis of patients with liver cancer; secondly, the 5-year recurrence and metastasis rate after liver cancer resection is as high as 40% to 70%. Existing diagnosis and t...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6886C12N15/11C12N15/12C40B40/06
CPCC12Q1/6886C40B40/06C12Q2600/154
Inventor 彭勇飞韩晓亮郭媛媛魏闯连明王小奇杨亚东
Owner BIOCHAIN BEIJING SCI & TECH
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com