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Marker and probe composition for breast cancer screening and application of marker and probe composition

A marker and composition technology, which is applied in the field of probe compositions and markers for breast cancer screening, can solve the problems of difficulty, missed detection of patients with small breasts, poor resolution of dense breast lesions, etc. Effects of Sensitivity and Accuracy

Pending Publication Date: 2022-04-19
BIOCHAIN BEIJING SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

If the radiation is large, the resolution of dense breast lesions is poor, and there is a possibility of missed detection for patients with small breasts
Moreover, Asian women are different from European and American women. The breasts of Asian women are relatively small, and most of them are "dense" breasts. For the inspection of such breasts, traditional mammography is relatively difficult.

Method used

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  • Marker and probe composition for breast cancer screening and application of marker and probe composition
  • Marker and probe composition for breast cancer screening and application of marker and probe composition
  • Marker and probe composition for breast cancer screening and application of marker and probe composition

Examples

Experimental program
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Effect test

preparation example Construction

[0184] The preparation of the chip is mainly based on a glass sheet or a silicon sheet as a carrier, and oligonucleotide fragments or cDNAs are arranged on the carrier in sequence as probes by means of in situ synthesis and micro-array.

[0185] The chip of the present invention is based on the signal detection of DNA sequence hybridization after bisulfite treatment. Bisulfite treatment is to change unmethylated cytosine into uracil, while methylated cytosine remains unchanged. Change, then convert uracil to thymine, and finally carry out chip hybridization; finally judge the type of base added according to the fluorescent color, and then determine whether the site is methylated.

[0186] The present invention provides a method for breast cancer screening, which comprises:

[0187] detect the methylation level of the marker, and

[0188] The risk of the subject suffering from breast cancer is judged based on the methylation level, and the gene corresponding to the marker is s...

Embodiment 1

[0192] Example 1 Screening markers

[0193] 1) Sample collection: Download the 450k methylation microarray cancer tissue data in TCGA, involving a total of 7,769 cancer tissue samples of 26 types of tumors, including adrenocortical carcinoma (80), bladder urothelial carcinoma (409), acute myeloid Leukemia (140), brain low-grade glioma (654), breast cancer (740), cervical cancer (286), colorectal cancer (348), esophageal cancer (183), uveal melanoma (80), head and neck squamous Stem cell carcinoma (527), kidney cancer (660), liver cancer (377), lung adenocarcinoma (425), lung squamous cell carcinoma (372), diffuse large B-cell lymphoma (29), ovarian serous cystadenocarcinoma ( 10), pancreatic cancer (184), mesothelioma (116), prostate cancer (488), skin melanoma (104), sarcoma (117), gastric cancer (397), testicular cancer (134), thymus cancer (94) , thyroid cancer (506), endometrial cancer (309). For healthy people, Boercheng collected the plasma of 38 healthy people, and th...

Embodiment 2

[0360] 6 cases of human samples (S1-3 are samples from healthy people, S4-6 are samples from breast cancer patients), using the methylation marker detection method of the application, collecting peripheral blood according to the method of Example 1; building a library, and passing Sequencing on the Illumina platform; the sequencing data is subjected to the above-mentioned bioinformatics analysis process to obtain the methylation level of each marker, and predict the patient's disease condition according to the threshold of each marker. If the threshold is exceeded, it is a cancer sample, if Below the threshold is a healthy sample, the specific results are shown in Table 19:

[0361] Among the interpretation results, 0 represents the classification as normal, that is, healthy; 1 represents the classification as abnormal, that is, tumor.

[0362] Table 19 Methylation values ​​and interpretation results of samples

[0363]

[0364]

[0365] In summary, the inventors of the...

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Abstract

The invention discloses a marker and a probe composition for breast cancer screening and application of the marker and the probe composition. The marker is selected from any one of 14 markers. According to the present invention, with the application of the marker, the methylation state of the gene can be sensitively and specifically detected so as to be used for the detection of the peripheral blood free DNA, and the composition is used for the screening of the asymptomatic population in the non-invasive manner so as to reduce the harm caused by the invasive detection; the composition has higher sensitivity and accuracy, and can realize real-time monitoring.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a marker for breast cancer screening, a probe composition and applications thereof. Background technique [0002] Breast cancer is a malignant tumor that occurs in the glandular epithelial tissue of the breast, and is the most common malignant tumor in women. The global incidence of breast cancer has been on the rise since the late 1970s. 1 in 8 women in the U.S. will develop breast cancer in her lifetime. China is not a country with a high incidence of breast cancer, but it should not be optimistic. In recent years, the growth rate of the incidence of breast cancer in my country is 1 to 2 percentage points higher than that of countries with a high incidence. Breast cancer has become a major public health problem in the current society. Since the 1990s, the mortality rate of breast cancer in the world has shown a downward trend. The reasons for this are: firstly, the development o...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6886C12Q1/6827C12Q1/6874C12N15/11
CPCC12Q1/6886C12Q1/6827C12Q1/6874C12Q2600/154C12Q2537/164C12Q2531/113C12Q2549/125C12Q2563/107C12Q2565/501
Inventor 连明韩晓亮王小奇杨亚东田继超刘栓平叶志海
Owner BIOCHAIN BEIJING SCI & TECH
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