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Marker and probe composition for colorectal cancer screening and application of marker and probe composition

A technology of colorectal cancer and markers, applied in the biological field, can solve the problems of lack of non-invasive diagnostic methods, achieve high sensitivity and accuracy, and reduce the effect of harm

Pending Publication Date: 2022-04-26
BIOCHAIN BEIJING SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Early detection is the key to improving patient survival and cure rates, but accurate non-invasive diagnostic methods are currently lacking

Method used

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  • Marker and probe composition for colorectal cancer screening and application of marker and probe composition
  • Marker and probe composition for colorectal cancer screening and application of marker and probe composition
  • Marker and probe composition for colorectal cancer screening and application of marker and probe composition

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0263] The preparation of the chip is mainly based on a glass sheet or a silicon sheet as a carrier, and oligonucleotide fragments or cDNAs are arranged on the carrier in sequence as probes by means of in situ synthesis and micro-array.

[0264] The chip of the present invention is based on the signal detection of DNA sequence hybridization after bisulfite treatment. Bisulfite treatment is to change unmethylated cytosine into uracil, while methylated cytosine remains unchanged. Change, then convert uracil to thymine, and finally carry out chip hybridization; finally judge the type of base added according to the fluorescent color, and then determine whether the site is methylated.

[0265] The present invention provides a method for colorectal cancer screening, which comprises:

[0266] detect the methylation level of the marker, and

[0267] The risk of the subject suffering from colorectal cancer is judged based on the methylation level, and the corresponding gene of the mar...

Embodiment 1

[0271] Example 1 Screening markers

[0272] 1) Sample collection: Download the 450k methylation microarray cancer tissue data in TCGA, involving a total of 7,769 cancer tissue samples of 26 types of tumors, including adrenocortical carcinoma (80), bladder urothelial carcinoma (409), acute myeloid Leukemia (140), brain low-grade glioma (654), breast cancer (740), cervical cancer (286), colorectal cancer (348), esophageal cancer (183), uveal melanoma (80), head and neck squamous Stem cell carcinoma (527), kidney cancer (660), liver cancer (377), lung adenocarcinoma (425), lung squamous cell carcinoma (372), diffuse large B-cell lymphoma (29), ovarian serous cystadenocarcinoma ( 10), pancreatic cancer (184), mesothelioma (116), prostate cancer (488), skin melanoma (104), sarcoma (117), gastric cancer (397), testicular cancer (134), thymus cancer (94) , thyroid cancer (506), endometrial cancer (309). For healthy people, Boercheng collected the plasma of 38 healthy people, and th...

Embodiment 2

[0442] 6 human samples (S1-3 are samples from healthy people, S4-6 are samples from patients with colorectal cancer), using the methylation marker detection method of the present application, collecting peripheral blood according to the method in Example 1; building a database, and Sequencing through the Illumina platform; the sequencing data is analyzed through the above-mentioned bioinformatics process to obtain the methylation level of each marker, and predict the patient’s disease status according to the threshold of each marker. If the threshold is exceeded, it is a cancer sample, If it is lower than the threshold, it is a healthy sample, and the specific results are shown in Table 19:

[0443] Among the interpretation results, 0 represents the classification as normal, that is, healthy; 1 represents the classification as abnormal, that is, tumor.

[0444] Table 19 Methylation values ​​and interpretation results of samples

[0445]

[0446]

[0447] To sum up, the ...

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Abstract

The invention discloses a marker and a probe composition for colorectal cancer screening and application of the marker and the probe composition. The marker is selected from any one of 28 markers. According to the present invention, with the application of the marker, the methylation state of the gene can be sensitively and specifically detected so as to be used for the detection of the peripheral blood free DNA, and the composition is used for the screening of the asymptomatic population in the non-invasive manner so as to reduce the harm caused by the invasive detection; the composition has higher sensitivity and accuracy, and can realize real-time monitoring.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a marker for screening colorectal cancer, a probe composition and applications thereof. Background technique [0002] Colorectal cancer is one of the most common cancers in China. According to the latest data released by the National Cancer Center in 2018, the number of new cases of colorectal cancer in my country reached 370,000 in 2014, accounting for about 26% of the world's total; ranked third. The cure rate for early colorectal cancer is 90%. The current clinically effective screening method is colonoscopy, but this invasive screening method is not subject to customer compliance. Early detection is the key to improving patient survival and cure rates, but accurate non-invasive diagnostic methods are currently lacking. At present, DNA methylation has been proved to be tissue-specific, which can be used for early cancer detection, and can be traced to the primary tumor site acc...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6886C12N15/11
CPCC12Q1/6886C12Q2600/154
Inventor 吴宁宁韩晓亮连明王小奇刘栓平魏闯杨亚东
Owner BIOCHAIN BEIJING SCI & TECH
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