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Marker and probe composition for thyroid cancer screening and application of marker and probe composition

A technology of thyroid cancer and markers, applied in the biological field, can solve problems such as over-medication, delayed surgery opportunities, and affecting thyroid function, achieving high sensitivity and accuracy, and reducing harm

Pending Publication Date: 2022-05-17
BIOCHAIN BEIJING SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Clinically, different ultrasound doctors may obtain different benign and malignant diagnosis results for the same nodule ultrasound image, which will eventually lead to over-medication or delay the best chance of surgery
In addition, high false-positive results from ultrasound reduce compliance with population-based screening because thyroid cancer resection scars the patient's neck and affects thyroid function

Method used

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  • Marker and probe composition for thyroid cancer screening and application of marker and probe composition
  • Marker and probe composition for thyroid cancer screening and application of marker and probe composition
  • Marker and probe composition for thyroid cancer screening and application of marker and probe composition

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0128] The preparation of the chip is mainly based on a glass sheet or a silicon sheet as a carrier, and oligonucleotide fragments or cDNAs are arranged on the carrier in sequence as probes by means of in situ synthesis and micro-array.

[0129] The chip of the present invention is based on the signal detection of DNA sequence hybridization after bisulfite treatment. Bisulfite treatment is to change unmethylated cytosine into uracil, while methylated cytosine remains unchanged. Change, then convert uracil to thymine, and finally carry out chip hybridization; finally judge the type of base added according to the fluorescent color, and then determine whether the site is methylated.

[0130] The present invention provides a method for thyroid cancer screening, which comprises:

[0131] detect the methylation level of the marker, and

[0132] The risk of the subject suffering from thyroid cancer is judged based on the methylation level, and the marker is selected from one of the ...

Embodiment 1

[0136] Example 1. Screening markers

[0137] 1) Sample collection: Download the 450k methylation microarray cancer tissue data in TCGA, involving a total of 7,769 cancer tissue samples of 26 types of tumors, including adrenocortical carcinoma (80), bladder urothelial carcinoma (409), acute myeloid Leukemia (140), brain low-grade glioma (654), breast cancer (740), cervical cancer (286), colorectal cancer (348), esophageal cancer (183), uveal melanoma (80), head and neck squamous Stem cell carcinoma (527), kidney cancer (660), liver cancer (377), lung adenocarcinoma (425), lung squamous cell carcinoma (372), diffuse large B-cell lymphoma (29), ovarian serous cystadenocarcinoma ( 10), pancreatic cancer (184), mesothelioma (116), prostate cancer (488), skin melanoma (104), sarcoma (117), thyroid cancer (397), testicular cancer (134), thymus cancer (94 ), thyroid cancer (506), endometrial cancer (309). For healthy people, Boercheng collected plasma from 38 healthy people, and the...

Embodiment 2

[0154] Example 2. Sample preparation, library building and verification methods

[0155] For a marker obtained in Example 1 that can distinguish thyroid cancer from healthy people, it was verified in a plasma sample, and the specific detection method was as follows:

[0156] 1.1.cfDNA extraction and purification

[0157] 1.1.1. Plasma sample preparation:

[0158] Centrifuge the blood sample at 2000 g for 10 min at 4°C and transfer the plasma to a new centrifuge tube. Centrifuge the plasma sample at 16000g for 10min at 4°C, and proceed to the next step according to the type of collection tube used. The type of collection tube used in this experiment is other.

[0159] Table 1

[0160]

[0161] 1.1.2. Lysis and conjugation

[0162] 1.1.2.1. Prepare the binding solution / bead mixture according to the table below and mix thoroughly.

[0163] Table 2

[0164]

[0165] Add an appropriate volume of plasma sample.

[0166] 1.1.2.2. Thoroughly mix the plasma sample and bind...

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Abstract

The invention discloses a marker and probe composition for thyroid cancer screening and application of the marker and probe composition. The marker is selected from any one of five markers. According to the present invention, with the application of the marker, the methylation state of the gene can be sensitively and specifically detected so as to be used for the detection of the peripheral blood free DNA, and the composition is used for the screening of the asymptomatic population in the non-invasive manner so as to reduce the harm caused by the invasive detection; the composition has higher sensitivity and accuracy, and can realize real-time monitoring.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a marker for thyroid cancer screening, a probe composition and applications thereof. Background technique [0002] Thyroid cancer is the most common malignant tumor of the endocrine system, accounting for about 1.5% of systemic malignant tumors. The average age of young adults with frequent thyroid cancer is about 40, and the ratio of male to female is about 1.0 to 2.5-3.0. At present, the incidence of thyroid cancer in the world is 3.1 / 100,000 males and 10.1 / 100,000 females, and it is increasing year by year with an annual increase of 4%, ranking ninth in the incidence of new cancers, and there is about 1 case of thyroid cancer in every 20 people. , is currently the fourth most frequently occurring malignant tumor in women, and the ninth new incidence rate in men, with a relatively low mortality rate. [0003] According to cancer classification statistics, the main reason for the ...

Claims

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Application Information

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IPC IPC(8): C12Q1/6886C12Q1/6837C12N15/11
CPCC12Q1/6886C12Q1/6837C12Q2600/154C12Q2535/122C12Q2531/113
Inventor 关晋霞韩晓亮连明王小奇郭媛媛张聪杨亚东
Owner BIOCHAIN BEIJING SCI & TECH
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