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Online solid-phase microextraction and high-performance liquid chromatography combined detection method for novel benzodiazepine drugs

A technology of high performance liquid chromatography and benzodiazepines, which is applied in the field of analytical chemistry, can solve problems such as endangering the health of consumers, achieve high-efficiency enrichment extraction and high-sensitivity online detection, improve extraction efficiency, and enhance π-π The effect of accumulation

Active Publication Date: 2022-04-29
FUZHOU UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

In addition, the illegal addition of BZD in tranquilizing health care products will seriously endanger the health of consumers

Method used

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  • Online solid-phase microextraction and high-performance liquid chromatography combined detection method for novel benzodiazepine drugs
  • Online solid-phase microextraction and high-performance liquid chromatography combined detection method for novel benzodiazepine drugs
  • Online solid-phase microextraction and high-performance liquid chromatography combined detection method for novel benzodiazepine drugs

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Embodiment

[0031] Step 1. Preparation of covalent organic framework surface functionalized solid phase microextraction monolithic column:

[0032] 1. Preparation of poly(glycidyl methacrylate-co-ethylene glycol dimethacrylate) monolithic column:

[0033] 1) Cleaning of empty stainless steel tube column: Connect a stainless steel empty column with a length of 33 mm and an inner diameter of 2.1 mm to a liquid phase pump, and wash it with chromatographically pure methanol at a flow rate of 1.0 mL / min for 20 minutes to remove the residue on the inner wall of the stainless steel tube. Impurities were removed, and dried in an oven at 60°C for 2 hours.

[0034] 2) In-tube free radical copolymerization: 300 mg glycidyl methacrylate, 100 mg ethylene glycol dimethacrylate, 120 mg N,N-dimethylformamide, 480 mg 1,4-butanediol, After mixing 1.2 mg of azobisisobutyronitrile with a vortex mixer, sonicate for 15 minutes at room temperature (ultrasonic frequency is 40 kHz, ultrasonic power is 100 W). T...

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Abstract

The invention discloses an online solid phase microextraction and high performance liquid chromatography combined detection method for novel benzodiazepine drugs. According to the invention, a covalent organic framework surface functionalized solid-phase microextraction monolithic column is used as a solid-phase microextraction column, and an in-tube solid-phase microextraction-high performance liquid chromatography on-line combined system is combined to establish the benzodiazepine novel drug on-line solid-phase microextraction and high performance liquid chromatography combined detection method. The hydrophobic interaction and pi-pi accumulation action between the covalent organic framework on the surface of the monolithic column and the benzodiazepine compounds are utilized, and efficient enrichment extraction and high-sensitivity online detection of trace benzodiazepine compounds in actual body fluid samples and beverage samples are achieved. The method is simple, the process is ingenious, required instruments are high in popularization degree and easy to popularize, and the requirement for efficient detection of benzodiazepine novel drugs is met.

Description

technical field [0001] The invention belongs to the field of analytical chemistry, and in particular relates to an on-line solid phase microextraction high performance liquid chromatography detection method for novel benzodiazepine drugs. Background technique [0002] Benzodiazepines (BZDs) are a class of central nervous system depressants with anti-anxiety, anti-convulsant, anti-muscle relaxation, and sedative and stable effects. The mechanism of action of BZD is to play a central nervous system inhibitory effect by enhancing the neurotransmission function and synaptic inhibitory effect of γ-aminobutyric acid. Excessive use of BZD can cause side effects such as dizziness, drowsiness, constipation, cardiopulmonary system diseases, and memory loss. In severe cases, it can cause addiction and even death. Due to BZDs' low cost, easy availability, and rapid onset of action, drug abuse is on the rise. In addition, the illegal addition of BZD in tranquilizing health care product...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N30/06G01N30/54G01N30/74
CPCG01N30/02G01N30/06G01N30/54G01N30/74
Inventor 王家斌孙健
Owner FUZHOU UNIVERSITY
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