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Chlamydia trachomatis/gonococcus/mycoplasma genitalium antigen combined detection kit and preparation method thereof

A technology for Chlamydia trachomatis and Mycoplasma genitalium, applied in measuring devices, immunoassays, biological material analysis, etc., can solve the problems of radioisotope contamination, complicated operation, poor sensitivity, etc., and achieve strong repeatability, strong specificity, and high sensitivity Effect

Pending Publication Date: 2022-04-29
北京泰格科信生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Its disadvantages: high cost, complicated operation, contamination by radioactive isotopes, etc.
Although the smear method is simple and fast, it has poor sensitivity; the culture method has high sensitivity but takes a long time; although the molecular biology technique has good sensitivity and specificity and is short in time, it requires expensive equipment and professional expertise. Technical personnel, which have a relatively large restrictive effect on its clinical implementation

Method used

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  • Chlamydia trachomatis/gonococcus/mycoplasma genitalium antigen combined detection kit and preparation method thereof
  • Chlamydia trachomatis/gonococcus/mycoplasma genitalium antigen combined detection kit and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Chlamydia trachomatis / Neisseria gonorrhoeae / Mycoplasma genitalium antigen combined detection kit, refer to Figure 1-2 , comprising three kinds of detection cards for respectively measuring Chlamydia trachomatis / gonococcus / mycoplasma genitalium antigen, said detection card comprising bottom plate 5, sample adding plate 1, latex binding pad 2, nitrocellulose membrane 3 and absorbent paper 4, said sample adding plate 1. Latex bonding pad 2, nitrocellulose membrane 3 and absorbent paper 4 are connected end to end in sequence and fixed on the bottom plate 5.

[0036] The latex binding pad 2 is immobilized with the first latex microsphere-labeled Chlamydia trachomatis / gonococcus / mycoplasma genitalium specific antibody and the second latex microsphere-labeled streptavidin, and the first latex microsphere is red The polystyrene microspheres have a particle diameter of 300nm; the second latex microspheres are blue polystyrene microspheres with a particle diameter of 300nm. ...

Embodiment 2

[0046] The method for using the chlamydia trachomatis / gonococcus / mycoplasma genitalium antigen combined detection kit is to apply the chlamydia trachomatis / gonococcus / mycoplasma genitalium antigen combined detection kit described in Example 1, comprising the following steps:

[0047] S1, perform swab sample processing on the female cervix, then add an appropriate amount of sample processing solution A to the sampling swab, rotate the swab 15 times and let it stand for 2 minutes, then add an appropriate amount of sample processing solution B dropwise, rotate the swab 15 times and then let it stand for 2 minutes. Leave it for 1 min, squeeze the swab along the tube wall, take out the swab and discard it, and obtain the sample solution; the sample treatment solution A is 0.2M NaOH solution, and the sample treatment solution B is 0.2M HCl solution;

[0048] S2, add the sample liquid dropwise to the sample plate of the test card, observe and judge the result within 15-25 minutes;

...

Embodiment 3

[0058] The method for using the chlamydia trachomatis / gonococcus / mycoplasma genitalium antigen combined detection kit is to apply the chlamydia trachomatis / gonococcus / mycoplasma genitalium antigen combined detection kit described in Example 1, comprising the following steps:

[0059] S1, perform swab sample processing on the female cervix, then add an appropriate amount of sample processing solution A to the sampling swab, rotate the swab 15 times and let it stand for 2 minutes, then add an appropriate amount of sample processing solution B dropwise, rotate the swab 15 times and then let it stand for 2 minutes. Leave it for 1 min, squeeze the swab along the tube wall, take out the swab and discard it, and obtain the sample solution; the sample treatment solution A is 0.2M NaOH solution, and the sample treatment solution B is 0.2M HCl solution;

[0060] S2, add the sample liquid dropwise to the sample plate of the test card, observe and judge the result within 15-25 minutes;

...

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Abstract

The invention discloses a chlamydia trachomatis / gonococcus / mycoplasma genitalium antigen combined detection kit and a preparation method thereof, and relates to the field of detection kits. Comprising a detection card, the detection card comprises a bottom plate, a sample adding plate, a latex combination pad, a nitrocellulose membrane and absorbent paper, and the sample adding plate, the latex combination pad, the nitrocellulose membrane and the absorbent paper are sequentially connected end to end and are fixed on the bottom plate; a first latex microsphere-labeled chlamydia trachomatis / gonococcus / mycoplasma genitalium specific antibody and a second latex microsphere-labeled streptavidin are fixed on the latex combination pad, and the nitrocellulose membrane is provided with a detection line coated with a chlamydia trachomatis / gonococcus / mycoplasma genitalium antibody and a quality control line coated with biotin-BSA (Bovine Serum Albumin). The detection kit provided by the invention can simultaneously complete antigen detection of chlamydia trachomatis / gonococcus / mycoplasma genitalium under the condition of only one-time sampling and sample treatment process, and is high in efficiency, strong in specificity and high in sensitivity.

Description

technical field [0001] The invention belongs to the field of detection kits, and in particular relates to a combined detection kit for chlamydia trachomatis / gonococcus / mycoplasma genitalium antigen and a preparation method thereof. Background technique [0002] Chlamydia trachomatis is an intracellular parasitic microorganism that can invade the eyes, reproductive tract and other organs. It is a common sexually transmitted disease. In addition to causing genitourinary tract infections, it is also a risk factor for increasing HIV infection one. [0003] Neisseria gonorrhoeae (Neisseria gonorrhoeae.NG), commonly known as Neisseria gonorrhoeae, is a human parasitic microorganism with a shape similar to meningococcus. It mainly resides in white blood cells in the purulent secretions of acute urethritis and vaginitis. It is mainly transmitted sexually and only infects humans. Men often cause acute urethritis, while women generally show asymptomatic infection, but the germs can s...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/569G01N33/558G01N33/546
CPCG01N33/56927G01N33/56933G01N33/56911G01N33/558G01N33/54313G01N2469/10G01N2333/295G01N2333/30G01N2333/22
Inventor 段虎杨锋陈明杰
Owner 北京泰格科信生物科技有限公司
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