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A miniature fluorescence immunoassay analyzer and its image processing method

A fluorescence immunoassay and fluorescence image technology, which is applied in the field of miniature fluorescence immunoassay analyzer and its image processing, can solve the problems of blurred outline of fluorescence image, large error, and low detection result accuracy, so as to protect edge information, reduce influence, Clearly defined effect

Active Publication Date: 2022-06-24
南京长健生物科技有限公司
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Problems solved by technology

[0004] The purpose of the present invention is to provide a miniature fluorescent immunoassay analyzer and its image processing method, which solves the problems of blurred outlines of fluorescent images in the prior art, low accuracy of detection results, and large errors

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  • A miniature fluorescence immunoassay analyzer and its image processing method
  • A miniature fluorescence immunoassay analyzer and its image processing method
  • A miniature fluorescence immunoassay analyzer and its image processing method

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Embodiment Construction

[0028] It is easy to understand that, according to the technical solutions of the present invention, without changing the essential spirit of the present invention, those of ordinary skill in the art can propose various alternative structures and implementations. Therefore, the following specific embodiments and accompanying drawings are only exemplary descriptions of the technical solutions of the present invention, and should not be regarded as all of the present invention or as limitations or restrictions on the technical solutions of the present invention.

[0029] According to an embodiment of the present invention combined figure 1 and 2 Shows. A miniature fluorescence immunoassay analyzer comprises a casing, a card slot assembly, a light source assembly, an image acquisition module and a main control CPU arranged inside the casing, the card slot assembly is used to place the detection strip 3, and the light source assembly is used to generate The fluorescent excitatio...

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Abstract

The present invention proposes a miniature fluorescence immunoassay analyzer and an image processing method thereof, the method comprising: acquiring at least two frames of fluorescence images, performing filter processing respectively to obtain a first fluorescence image and a second fluorescence image; according to the fluorescence luminescence intensity curve, Obtain the first threshold; compare the pixel values ​​of corresponding pixels on the first fluorescence image and the second fluorescence image, and obtain the third fluorescence image according to the first threshold; process the third fluorescence image by using the maximum inter-class variance method to obtain The second threshold, and based on the second threshold, binarize and segment the third fluorescence image to obtain a binarized image; iteratively scan all pixels on the binarized image, mark similar pixels therein and record them in the marker matrix , to obtain the target area; calculate the average gray value of the target area, and obtain the concentration of the antigen or antibody in the sample according to the proportional relationship between the gray value and the intensity of the fluorescent signal. The invention reduces detection errors and ensures the accuracy of detection results.

Description

technical field [0001] The invention relates to the field of immunochromatographic detection, in particular to a miniature fluorescence immunoassay analyzer and an image processing method thereof. Background technique [0002] Fluorescence immunoassay analyzer is an emerging immunoassay instrument based on chromatography technology and antigen-antibody specific immune response. The fluorescence immunoassay analyzer uses the strip fiber chromatography material with the detection line (T line) and the control line (C line) fixed as the chromatography test paper, and the detection sample (such as serum, plasma, whole blood, saliva, etc.) Moving on the chromatographic test paper, and in the process of moving, the analyte in the detection sample is combined with the fluorescently labeled primary antibody to form the analyte-primary antibody-fluorescein structure, and continues to move forward, passing through the T of the NC membrane. When the analyte-first antibody-fluorescein ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/64G01N33/53G06T7/00G06T5/00G06T5/20G06T7/11G06T7/136G06T7/90
CPCG01N21/6486G01N33/53G06T7/0012G06T5/002G06T5/20G06T7/11G06T7/136G06T7/90G06T2207/10064G06T2207/20192
Inventor 李刚贾俊玉
Owner 南京长健生物科技有限公司
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