Trimeric ubiquitin chain probe as well as synthesis method and application thereof

Abstract

The invention relates to a trimeric ubiquitin chain probe as well as a synthesis method and application thereof, and particularly, the trimeric ubiquitin chain probe has a structure as shown in a formula I-a or a formula I-b. The trimeric ubiquitin chain probe disclosed by the invention has higher simulation, has higher reliability when being used for researching related functions of the deubiquitinase, and can be further used for researching exo-cleavage and endo-cleavage selective mechanisms of hydrolysis of a polyubiquitin chain.

Description

technical field [0001] The invention relates to the field of synthesis and application of polyubiquitin chain probes, in particular to a trimeric ubiquitin chain probe and its synthesis method and application. Background technique [0002] Protein ubiquitination is one of the most important post-translational modifications in eukaryotes, and plays a decisive role in many diseases and physiological processes, such as proteasomal degradation and DNA damage repair. Ubiquitin is a post-translational modification protein containing 76 amino acids, with 7 lysines on it. At these lysine sites and N-terminal amino groups of ubiquitin, autoubiquitination also occurs, resulting in the formation of linear, K6-linked, K11-linked, K27-linked, K29-linked, K33-linked, K48 Various types of polyubiquitin chains linked by -link and k63, these ubiquitin chains perform related physiological functions in different cell signaling pathways due to differences in linking methods and structures. Th...

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Problems solved by technology

However, there is no report on the synthesis of fluorescent probes based on triubiquitin.

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