All-male breeding method for giant spiny frogs

Abstract

The invention provides a giant spiny frog all-male breeding method, and relates to the technical field of giant spiny frog artificial breeding. The method comprises the following steps: (1) dissolving trozole in absolute ethyl alcohol, uniformly stirring with feed to prepare 20-1000mg / Kg of poison bait, and storing in a refrigerator at 4 DEG C for later use; (2) the tadpoles are subjected to micro-running water breeding in the breeding pond, after the tadpoles are demolded and open, the tadpoles are fed with the poison bait prepared in the step (1), and the daily feeding amount is 11-15% of the weight of the tadpoles in the whole pond; and (3) 90 days after feeding the poison bait in the step (2), feeding normal feed until metamorphosis is completed. The method is efficient and safe, the gender proportion of the offspring of the species can be effectively made to deviate from the male gender, the male rate of the giant spiny froglets can be remarkably increased by feeding the feed containing letrozole with different concentrations, and meanwhile letrozole does not affect growth of metamorphosis froglets of the giant spiny froglets while increasing the male rate.

Description

technical field [0001] The invention belongs to the technical field of artificial breeding of spiny-breasted frog, and in particular relates to a method for breeding all-male spiny-breasted frog. Background technique [0002] The spinous frog (Quasipaa spinosa) is mainly distributed in the mountainous areas of southeastern China and northern Vietnam. It is a unique medicinal and edible animal in my country. It has high nutritional value and is known as the "king of frogs". In recent years, due to habitat destruction, extensive use of herbicides and other pesticides, and over-fishing, the wild resources of spiny frogs have been decreasing. At present, spiny frogs have been listed as "easy to catch" by the International Union for Conservation of Nature (IUCN) and China's Red List. endangered” species. Artificial breeding is one of the important methods to protect the resources of spiny-breasted frogs. At the same time, the economic performance of male individuals is better th...

AI Technical Summary

Problems solved by technology

Previous studies have found that feeding diets containing 17-αmethyltestosterone will significantly increase the male rate of the offspring of spiny-breasted frog tadpoles, the highest being only 66% (the male rate of the control group is 49.1%), and it will also cause the growth of spiny-breasted frog to be affected. Inhibition, mortality and deformity rates significantly increased

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