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RNAi interference fragment, interference carrier, preparation method and application thereof

A technology for interfering vectors and fragments, applied in the field of RNA interference fragments, can solve problems such as poor motility, expensive equipment, and low sperm count, and achieve the effects of increased male rate, improved chance of fertilization, and low cost

Active Publication Date: 2020-06-16
SHIHEZI UNIVERSITY
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  • Description
  • Claims
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Problems solved by technology

[0004] At present, according to the principle of the difference in DNA content of mammalian X and Y sperm, flow cytometry is used to separate X and Y sperm. The separation effect is good, and the purity can reach more than 90%, but the equipment is expensive and the separation speed is slow. Moreover, due to the low number of fertilized sperm and poor vitality, it is far from meeting the large demand for sexually controlled semen in production.

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  • RNAi interference fragment, interference carrier, preparation method and application thereof
  • RNAi interference fragment, interference carrier, preparation method and application thereof
  • RNAi interference fragment, interference carrier, preparation method and application thereof

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Embodiment Construction

[0036] In order to further explain the technical means and effects of the present invention to achieve the intended purpose of the invention, the following in conjunction with the accompanying drawings and preferred embodiments, a RNAi interference fragment, interference carrier and its preparation method and application proposed according to the present invention, Its specific implementation, structure, feature and effect thereof are described in detail as follows. In the following description, different "one embodiment" or "embodiment" do not necessarily refer to the same embodiment. Furthermore, the particular features, structures or characteristics of one or more embodiments may be combined in any suitable manner.

[0037] 1. The preparation method of the RNAi interference carrier provided by the invention is:

[0038] According to the mRNA sequence of the domestic cattle Zfx gene provided in GenBank, specific primers were designed to amplify the red deer Zfx gene. The se...

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Abstract

The invention provides an RNAi interference fragment, an interference carrier and preparation methods and application thereof. The RNAi interference fragment and the interference carrier aim at a Zfx gene. The RNAi interference fragment at least comprises the following first sequence or second sequence, wherein the first sequence is F:5'-TGTGA GGACT ACCTT ATGAT TTCAA GAGAA TCATA AGGTA GTCCT CACTT TTTTC-3', and the second sequence is R:5'-TCGAG AAAAA AGTGA GGACT ACCTT ATGAT TCTCT TGAAA TCATA AGGTA GTCCT CACA-3'. The RNAi interference fragment is used for interferring the Zfx gene on animal X chromosome. The interference fragment disclosed by the invention can effectively control animal gender.

Description

technical field [0001] The invention relates to the field of animal molecular genetics, in particular to an RNA interference fragment capable of silencing a gene related to X sperm production and an application thereof. Background technique [0002] Under natural conditions, the sex ratio of almost all mammals is close to 1:1, thus achieving uninterrupted and stable reproduction of offspring and achieving ecological balance under natural conditions. For the actual production of livestock, the ideal sex ratio is of great significance. Production enterprises using lactation and egg production traits hope to obtain more female individuals, while those with meat production and hair production traits have more obvious advantages in male individuals. Humans have always been interested in controlling the sex of animal offspring. At present, gender control is mainly carried out from two aspects: pre-fertilization and post-fertilization. The former uses sperm separation and then fe...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/113C12N15/85C12N15/66A01K67/027
CPCA01K67/0275A01K2207/05A01K2217/058A01K2227/10A01K2267/02C12N15/113C12N15/85C12N2310/14C12N2800/107
Inventor 贾斌李超程杜迎春
Owner SHIHEZI UNIVERSITY
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