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Method for sex determination of mammal

A technology for mammals and gender, applied in animal delivery, cells modified by the introduction of foreign genetic material, medical science, etc., can solve the problems of limited number of embryos for gender identification, genetic influence, limited number, etc., to reduce gender control Cost, economical and practical purity, and the effect of improving productive cattle

Inactive Publication Date: 2007-02-07
LAIYANG AGRI COLLEGE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] 1. Waste of resources
Half of the sperm in the separation of X and Y sperm are eliminated, resulting in a waste of resources
Embryo gender identification requires the removal of a small number of cells from implantable embryos that develop into blastocysts or morulae after fertilization, and DNA amplification in vitro to obtain male and female information, so as to achieve a technology that controls the sex of offspring. If the natural distribution of male and female is used Calculated according to the ratio, 50% of the embryos are eliminated, which again causes a waste of resources and economy
[0004] 2. Quantity is limited
①Internal fertilization can only obtain one good-bred offspring a year; ②Although in vitro fertilization can obtain most embryos of known gender, it is difficult to obtain good-bred offspring due to the influence of maternal genetics, because the process of in vitro fertilization requires the mother to be taken from a slaughterhouse. The ovaries of cattle, the ovaries taken from the slaughterhouse are basically to eliminate cows or low-quality cows, so the genetics of the offspring made of in vitro fertilization are greatly affected; ③The number of sex identification embryos obtained through superovulation is limited
[0005] 3. The price is expensive and not easy to be accepted by the common people

Method used

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  • Method for sex determination of mammal
  • Method for sex determination of mammal
  • Method for sex determination of mammal

Examples

Experimental program
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Embodiment 1

[0032]Example 1. Using Bovine Bone Marrow Mesenchymal Stem Cells to Control the Sex of Cattle

[0033] 1. Isolation of bovine bone marrow mesenchymal stem cells

[0034] 1. Isolation of bone marrow mesenchymal stem cells and their primary (first generation) culture

[0035] Isolate the femoral bone marrow of cattle (2-6 months old) under aseptic conditions, use a 5ml syringe with a No. Gently pipette and pass through a 200-mesh filter to make a single-cell suspension. The single cell suspension was washed 3 times with phosphate buffer solution (PBS-) without calcium and magnesium ions, and after resuspended, the cell suspension was gently added to a 4°C pre-cooled In a test tube of Percoll cell separation solution with a density of 1.082 (purchased from Sigma, USA, formula: Percoll stock solution 25.65mL+1.5moL / L NaCl solution 24.35mL), centrifuge at 80g for 30min to take the cloud-like single cell in the middle (the junction of the liquid surface) The nuclear cell layer wa...

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Abstract

the invention discloses a mammal sex controlling method, which is characterized by the following: adopting bone marrow mesenchyma stem cell as core supplier cell; moving in the mature, stoning and unfertilized egg cell; reconstructing; cultivating externally; proceeding embryo transplantation.

Description

technical field [0001] The present invention relates to a method for controlling the sex of mammals. Background technique [0002] There are two traditional ways of sex control, namely control before fertilization and control after fertilization. The former refers to the artificial intervention of sperm before fertilization, and the sex of the offspring is determined at the time of fertilization; the latter refers to the sex identification of fertilized eggs so that they can obtain offspring of the desired sex. The disadvantages of these two gender control methods are: [0003] 1. Waste of resources. In the separation of X and Y sperm, half of the sperm are eliminated, resulting in a waste of resources. Embryo gender identification requires the removal of a small number of cells from implantable embryos that develop into blastocysts or morulae after fertilization, and DNA amplification in vitro to obtain male and female information, so as to achieve a technology that cont...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/10A61D19/00
Inventor 董雅娟赵兴封纪武柏学进胡亮
Owner LAIYANG AGRI COLLEGE
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