A method for masculinization of Chinese giant salamander induced by high temperature

Abstract

The invention discloses a method for inducing the masculinization of andrias davidianus at high temperature. The method comprises the steps: A, determining the starting time of gonad differentiation of the andrias davidianus; and carrying out histological observation on gonad of the andrias davidianus in different growth stages by means of tissue slicing in combination with microscopic examination; B, determining the high-temperature lethal temperature of the andrias davidianus; and continuously treating young andrias davidianus breaking out of a membrane at different temperatures; C, identifying the phenotypic ratio of the andrias davidianus at different temperatures: treating the young andrias davidianus not subjected to sex differentiation at high temperature until the sex differentiation is completed, and then, when the young andrias davidianus is raised to be 1-1.5 years old at normal temperature, collecting gonads of treated fish fries, and carrying out histological identification on the phenotypic sex; and D, analyzing and identifying the expression of a high-temperature treated ovary by using a sex related gene. The method is easy and simple and convenient to operate, the temperature for high-temperature induction of the masculinization of the andrias davidianus is made clear, the problem that male parents effectively taking part in propagation are shorted due to smallsemen amount or relatively poor semen quality of most of male andrias davidianus in production at present is solved, and a technical means is provided for subsequent andrias davidianus sex control andall-male breeding.

Description

technical field [0001] The invention belongs to the technical field of aquaculture, and more specifically relates to a growth method for inducing masculinization of giant salamanders. Background technique [0002] The Chinese Giant Salamander (Andrias davidianus), also known as the "Salamander", is the largest existing freshwater amphibian in the world. According to historical records, wild giant salamanders are distributed in Sichuan, Hubei, Yunnan and other provinces in my country. Due to human hunting and habitat destruction, the population of wild giant salamanders has declined sharply. Chinese giant salamander is an economic animal with high edible and medicinal value. It has a unique flavor and has the effects of nourishing yin, tonifying kidney, nourishing blood and promoting qi (Zhang Shenhu, 2001), and is known as "ginseng in water" (Hou Jinhui et al., 2004). In order to protect and utilize giant salamander resources, since the 1960s, domestic scientific and techno...

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Problems solved by technology

[0004] The purpose of the present invention is to provide a method for high-temperature induced masculinization of giant salamander, which is easy to implement and easy to operate. By studying the influence of environmental factors (such as temperature) on gonad development and sex differentiation of giant salamander, it has been clarified that high-temperature induced giant salamander The temperature of masculinization has established a high temperature induced masculinization of giant salamanders, which solves the problem of lack of effective reproductive male parents due to the lack of spermatozoa or poor sperm quality in the current production of male giant salamanders, and a method for subsequent sex control and all-male breeding of giant salamanders. technical means

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