Method for preparing menstrual blood-derived mesenchymal stem cells

Abstract

The invention belongs to the technical field of cell separation and culture, and discloses a method for preparing menstrual blood-derived mesenchymal stem cells. According to the method, a menstrual blood sample is sieved by a 10-30-mesh sieve, blood clots and large tissues are removed, and the centrifugal effect is prevented from being influenced during subsequent centrifugation; on the basis of a traditional method, namely collection of the albuginea layer, the step of collecting the blood cell layer is added, so that target cells of different separation layer samples subjected to density gradient centrifugation can be recovered by using the traditional method and the method of collecting the blood cell layer at the same time; the recovery rate of primary target cells can be improved on the basis of an original traditional method, and later production and library building are facilitated; compared with a traditional separation method, the method has the advantages that the proliferation capacity of the cells prepared by the method in the later culture process is higher than that of the cells prepared by the traditional separation method, and the method plays a positive role in shortening the in-vitro culture time on the premise of ensuring the cell quality in the later period so as to realize that the cells of early generations are used as preparation products.

Description

technical field [0001] The invention belongs to the technical field of cell separation and culture, and in particular relates to a method for preparing menstrual blood-derived mesenchymal stem cells. Background technique [0002] Cells used in stem cell therapy include embryonic stem cells, induced pluripotent stem cells, and mesenchymal stem cells. Among them, mesenchymal stem cells (MSCs) have attracted the attention of researchers due to their high proliferation ability, self-renewal ability, mesodermal differentiation ability, and low immunogenicity. In recent years, mesenchymal stem cells have also shown great development potential in immune regulation and tissue regeneration. So far, researchers have discovered and isolated mesenchymal stem cells from different human tissues. The endometrium undergoes regular changes with the menstrual cycle, and the highly regenerative capacity of this tissue suggests that there may be stem cells in the endometrium that facilitate i...

AI Technical Summary

Problems solved by technology

[0004] The preparation of menstrual blood-derived mesenchymal stem cells in the prior art has the following problems: due to the influence of sample blood clots and viscosity, the effect of density gradient centrifugation is quite different, and it is impossible to efficiently and easily enrich menstrual blood-derived mesenchymal stem cells in different forms. mesenchymal stem cells, and the cell recovery rate of simply collecting buffy coat cells is low

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