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Method for rapidly screening human hsa-miR-576-3p and promoter binding target

A promoter and fast technology, applied in the field of biomedicine, can solve problems such as impracticability, predictive software and website without maintenance, etc., and achieve the effect of rapid screening and convenient research and use

Active Publication Date: 2022-05-31
THE FIRST AFFILIATED HOSPITAL OF WANNAN MEDICAL COLLEGE YIJISHAN HOSPITAL OF WANNAN MEDICAL COLLEGE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the existing technical solutions cannot be directly used for the combination of miRNA and the promoter target, and the existing prediction software and website are not maintained or cannot realize this function. There is an urgent need for a rapid screening of human hsa-miR-576 and promoter Subtarget combined approach for non-classical research

Method used

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Examples

Experimental program
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Embodiment

[0016] Example: a method for rapid screening of human hsa-miR-576-3p and promoter binding targets, the following steps are all completed on the computer terminal, as follows:

[0017] S1. Download and install samtools, bedtools, bedops and seedVicious software:

[0018] Download and install samtools

[0019] https: / / github.com / samtools / samtools / releases / download / 1.14 / samtools-1.14.tar.bz2

[0020] placed in the soft folder.

[0021] >tar-jxvfsamtools-1.14.tar.bz2

[0022] >cd / Users / Documents / soft / samtools-1.14

[0023] >. / configure--prefix= / Users / Documents / soft / samtools-1.14

[0024] >make

[0025] >make install

[0026] >echo'export PATH=$PATH:~ / Users / Documents / soft / samtools-1.14'>>~ / .bashrc

[0027] >source~ / .bashrc

[0028] Download and install bedtools

[0029] >cd~ / Users / Documents / soft

[0030] >wget

[0031] https: / / github.com / arq5x / bedtools2 / releases / download / v2.29.1 / bedtools-2.29.1.tar.gz

[0032] >tar-zxvfbedtools-2.29.1.tar.gz

[0033] >cdbedtools2

[...

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Abstract

The invention discloses a method for rapidly screening a binding target of human-derived hsa-miR-576-3p and a promoter, which comprises the following steps: downloading a newest human genome sequence, a corresponding annotation file and a human-derived hsa-miR-576-3p sequence file; extracting the chromosome length of the newest human genome sequence from the downloaded newest human genome sequence, and extracting the sequence of the promoter from the newest human genome sequence and the corresponding annotation file; and S2, screening a human-derived hsa-miR-576-3p sequence and the promoter sequence extracted in S2 by virtue of seedVicius software, and reserving the variety of a target which is completely matched with the miRNA seed sequence, so as to obtain a prediction result of mmu-miR-25-3p and the promoter target. According to the method disclosed by the invention, the existing data and software are utilized, and the binding target of the human source hsa-miR-576-3p and the promoter is quickly screened on a computer.

Description

technical field [0001] The invention belongs to the technical field of biomedicine, and specifically relates to a method for rapidly screening human hsa-miR-576-3p and a promoter binding target. Background technique [0002] MicroRNAs (miRNAs) are small, siRNA-like molecules encoded by higher eukaryotic genomes that degrade mRNAs or block their translation by base-pairing with target mRNA-directed silencing complexes (RISCs). miRNAs are quite conserved in species evolution. miRNAs found in plants, animals and fungi are only expressed in specific tissues and developmental stages. The tissue specificity and timing of miRNAs determine the functional specificity of tissues and cells, indicating that miRNAs play a role in cell growth. and play a variety of roles in the regulation of developmental processes. At present, more and more attention is paid to the research of microRNAs, which has great value for the diagnosis of many diseases. [0003] The promoter is a DNA sequence l...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G16B20/30
CPCG16B20/30
Inventor 蒋澜杨建课吕坤钟民王祖贞罗天乐汪乐瑶邓汉诺冯翔杜金锐
Owner THE FIRST AFFILIATED HOSPITAL OF WANNAN MEDICAL COLLEGE YIJISHAN HOSPITAL OF WANNAN MEDICAL COLLEGE