Dental pulp stem cell cryopreservation liquid and cryopreservation method

A technology of dental pulp stem cells and cryopreservation solution, applied in the field of cryopreservation solution, can solve problems such as toxicity, low cell recovery rate, high osmotic pressure, etc., and achieve the effects of easy operation, improved survival rate, and reduced damage

Pending Publication Date: 2022-06-07
张明新
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, dimethyl sulfoxide has a certain toxic effect on cells. Too high concentration will cause high osmotic pressure, resulting in low cell recovery rate.

Method used

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  • Dental pulp stem cell cryopreservation liquid and cryopreservation method

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Experimental program
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Effect test

Embodiment 1

[0029] A dental pulp stem cell cryopreservation solution, consisting of a basal medium and the following components added to the basal medium: glycerol, corn silk ultrafine powder, isosteviol, dextran-40, sodium hyaluronate, poly Vinylpyrrolidone; the final concentrations of each component in the medium are: glycerol 12 μg / mL, corn silk ultrafine powder 8 μg / mL, isosteviol 16 μg / mL, sodium hyaluronate 7 μg / mL, dextran-40 2 mg / mL, polyvinylpyrrolidone 25μg / mL; the particle size of corn silk ultrafine powder is 100-150 mesh.

[0030] A method for cryopreserving dental pulp stem cells, comprising the following steps:

[0031] (1) Preparation of cryopreservation solution: add glycerol, dextran-40, corn silk ultrafine powder, isosteviol, sodium hyaluronate, and polyvinylpyrrolidone to the basal medium, and mix well to obtain a cryopreservation solution for use;

[0032] (2) The P3 generation dental pulp stem cells in logarithmic growth phase were resuspended in the cryopreservatio...

Embodiment 2

[0035] A dental pulp stem cell cryopreservation solution, consisting of a basal medium and the following components added to the basal medium: glycerol, corn silk ultrafine powder, isosteviol, dextran-40, sodium hyaluronate, poly Vinylpyrrolidone; the final concentrations of each component in the medium are: glycerol 10 μg / mL, corn silk ultrafine powder 6 μg / mL, isosteviol 14 μg / mL, dextran-40 1 mg / mL, sodium hyaluronate 5μg / mL, polyvinylpyrrolidone 23μg / mL; the particle size of corn silk ultrafine powder is 100-150 mesh.

[0036] A method for cryopreserving dental pulp stem cells, comprising the following steps:

[0037] (1) Preparation of cryopreservation solution: add glycerol, dextran-40, corn silk ultrafine powder, isosteviol, sodium hyaluronate, and polyvinylpyrrolidone to the basal medium, and mix well to obtain a cryopreservation solution for use;

[0038] (2) The P3 generation dental pulp stem cells in logarithmic growth phase were resuspended with the cryopreservati...

Embodiment 3

[0041] A dental pulp stem cell cryopreservation solution, consisting of a basal medium and the following components added to the basal medium: glycerol, corn silk ultrafine powder, isosteviol, dextran-40, sodium hyaluronate, poly Vinylpyrrolidone; the final concentrations of each component in the medium are: glycerol 15 μg / mL, corn silk ultrafine powder 10 μg / mL, isosteviol 18 μg / mL, dextran-405 mg / mL, sodium hyaluronate 10μg / mL, polyvinylpyrrolidone 29μg / mL; the particle size of corn silk ultrafine powder is 100-150 mesh.

[0042] A method for cryopreserving dental pulp stem cells, comprising the following steps:

[0043](1) Preparation of cryopreservation solution: add glycerol, dextran-40, corn silk ultrafine powder, isosteviol, sodium hyaluronate, and polyvinylpyrrolidone to the basal medium, and mix well to obtain a cryopreservation solution for use;

[0044] (2) Resuspend the P3 generation dental pulp stem cells in logarithmic growth phase using the cryopreservation sol...

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Abstract

The invention discloses a dental pulp stem cell cryopreservation solution which comprises a basic culture medium and the following components added into the basic culture medium: glycerol, corn stigma superfine powder, isosteviol, dextran 40, sodium hyaluronate and polyvinylpyrrolidone. The dental pulp stem cell cryopreservation liquid provided by the invention is not added with dimethyl sulfoxide. Wherein the addition of the corn stigma superfine powder and the isosteviol reduces the dosage of the glycerol, and the glycerol and the isosteviol synergistically act to protect the dental pulp stem cells in the cryopreservation process, so that the damage to the dental pulp stem cells in the cryopreservation process is reduced, the motility rate of the dental pulp stem cells after cryopreservation is improved, and the preservation time of the dental pulp stem cells is prolonged. The invention further provides a cryopreservation method of the dental pulp stem cells, the cryopreservation liquid is adopted, operation is easy, and batch preservation of the dental pulp stem cells is facilitated.

Description

technical field [0001] The invention relates to a cryopreservation solution, in particular to a dental pulp stem cell cryopreservation solution and a cryopreservation method. Background technique [0002] The pulp tissue is located in the pulp cavity inside the tooth and is the only soft tissue in the tooth tissue. In 2000, Gronthos et al., through the study of human dental pulp cells, found a cell with a very similar immune phenotype and the ability to form mineralized nodules with bone marrow mesenchymal stem cells. The cells are spindle-shaped and self-renewing. And multi-directional differentiation, has a strong cloning ability. These fibroblasts isolated from dental pulp tissue are called dental pulp stem cells. [0003] Dental pulp stem cells have the potential of multi-directional differentiation. In addition to the cells that can form mineralized nodules, they can also differentiate into fat, bone, cartilage, muscle, vascular endothelial, liver, nerve and other cel...

Claims

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Application Information

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IPC IPC(8): A01N1/02
CPCA01N1/0221A01N1/0226A01N1/0284
Inventor 张明新顾磊
Owner 张明新
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