Novel lateral flow chromatography test strip based on molecular imprinting, preparation method and application of novel lateral flow chromatography test strip in detection of small molecular substance sulfur diethylene glycol

Abstract

The invention relates to a novel lateral flow chromatography test strip based on molecular imprinting, a preparation method and a method for instantly detecting a small molecular substance sulfur diethylene glycol. The lateral flow chromatography test strip comprises a sample pad, a combination pad, a nitrocellulose membrane, a water absorption pad and a back plate. Gold nanoparticles (gold nanoparticles) are loaded on the combination pad, the small molecular substance sulfur diglycol and the gold nanoparticles are combined through Au-S bonds, a molecularly imprinted polymer of the small molecular substance sulfur diglycol is synthesized to serve as a detection line material, recognition and capture of the small molecular substance sulfur diglycol on the test strip are achieved, metallothionein is designed to serve as a quality control line material, and the detection line is used for detecting the detection line. The gold nanoparticles and the metallothionein can be combined through Au-S bonds. The designed lateral flow chromatography test strip can visually judge whether the small molecular substance sulfur diethylene glycol is contained or not by detecting a small amount of urine samples, and quantitative detection is achieved through image J analysis. The novel lateral flow chromatography test strip has the advantages of high sensitivity, strong specificity and the like when being used for detecting small molecular substances, namely sulfur diethylene glycol.

Description

technical field [0001] The invention belongs to the technical field of novel lateral flow chromatography test strips, and in particular relates to a novel lateral flow chromatography test strip based on molecular imprinting and its application in the detection of small molecular substance thiodiglycol. Background technique [0002] Lateral flow chromatography analysis technology is widely used in the detection of biomarkers in blood, urine, saliva and other samples due to its advantages of rapidity, sensitivity, low price and no need for professional operation. It is used in disease diagnosis, food and environmental monitoring. play an important role in other fields. In resource-poor areas, such as developing countries and remote areas, lateral flow chromatography analysis technology can greatly replace expensive and large-scale instruments and equipment, and realize on-site instant detection. [0003] At present, most traditional lateral flow chromatography analysis techni...

AI Technical Summary

Problems solved by technology

The small molecular substance thiodiglycol can also be analyzed by GC-MS, but the peak shape is not ideal. When the analysis concentration is lower than 1ppm, derivatization is required, and the small molecular substance thiodiglycol needs to be modified to be more volatile and Only less polar derivatives can be quantitatively and qualitatively analyzed by GC-MS

These detection methods not only require expensive instruments and reagents, but also require professionals to operate, it is difficult to realize the on-site and instant detection of small molecular substances thiodiglycol

Images