Bacillus velezensis ZLP-101 and application thereof

A technology of ZLP-101 and Bacillus velaisi, which is applied in the fields of application, bacteria, and fungicides, can solve the problems of killing natural enemies and beneficial microorganisms, environmental and human health hazards, and the increase of pest resistance, and achieves good application The effects of foreground, environmental friendliness and fast reproduction speed

Active Publication Date: 2022-07-08
BIOLOGY INST OF HEBEI ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003]Currently, chemical control is mainly used for diseases. Conventional chemical pesticide control not only kills a large number of natural enemies and beneficial microorganisms, destroys the ecological balance, but also sharply increases the resistance of pests ;Pesticide residues have caused great harm to the environment and human health

Method used

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  • Bacillus velezensis ZLP-101 and application thereof
  • Bacillus velezensis ZLP-101 and application thereof
  • Bacillus velezensis ZLP-101 and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Example 1 Isolation and screening of Bacillus velesi ZLP-101

[0037] (1) Taking soil samples: Collect soil samples from Xingtai, Hebei, each 50-100g; put them into sterilized kraft paper bags, seal the bag, and record the sampling location, environment and date.

[0038] (2) Bacillus isolation and purification: Soil samples were isolated by plate dilution method on PB medium. The specific operation is as follows: Accurately weigh 10g of soil sample, add it to a 90mL 0.9% physiological saline conical flask with glass beads, shake at 180r / min for 30min, make the soil sample evenly distributed and make a soil suspension with a concentration of 10 -1 ; successively diluted to a concentration of 10 -5 , 10 -6 , 10 -7 . In a constant temperature water bath at 80 °C for 30 min, 100 μL of each dilution was coated on PB plates, and the plates were incubated upside down in a constant temperature incubator at 30 °C for 48 h. Single colonies of different shapes were picked an...

Embodiment 2

[0042] Example 2 Identification of Bacillus velesi ZLP-101

[0043] According to the experimental methods in the Handbook of Systematic Identification of Common Bacteria, the morphological, physiological and biochemical identification of the strains with strong insecticidal and disease prevention effects was carried out. The strain was cultured on PB medium plate at 30±1℃ for 14-16h, and it was observed that the colony numbered BLS2 was round, white in color, dull in color, rough in surface and irregular in edge, and the cell was rod-shaped. The spores are oval in shape (e.g. Figure 1 to Figure 3 shown). Its physiological and biochemical identification indicators are shown in Table 1.

[0044] Table 1 Physiological and biochemical properties of strain ZLP-101

[0045]

[0046] Note: "+" means positive; "-" means negative.

[0047] Through morphological observation and physiological and biochemical experiments, it was determined that the strain numbered BLS2 was Bacillu...

Embodiment 3

[0049] Example 3 Insecticidal effect of Bacillus velesi ZLP-101 on Hemipteran pests such as aphids

[0050] Bacillus velesi ZLP-101 was fermented and cultured. NB liquid medium was used, and the formula was: beef extract 5g / L, peptone 10g / L, NaCl 5g / L, glucose 10g / L, pH 7.0. After inoculation, they were placed in a shaking incubator at 180 r / min and 32 °C for 48 h. The fermentation broth was diluted to 10-fold, 20-fold and 50-fold, and treated with clean water as a control. The food used in the insecticidal experiment of the bean aphid (Aphiscraccivora Koch) was the young stem of broad bean, the food used in the insecticidal experiment of the cotton aphid (Aphis gossypii) was the young cotton stem, and the food used in the insecticidal experiment of the cabbage aphid (Brevicoryne brassicae) was the young leaves of cabbage The food used in the insecticidal experiment of Bemisia tabaci was tomato leaves. These four kinds of food were soaked in the above-mentioned liquid for 10...

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Abstract

The invention relates to a bacillus velezensis ZLP-101 with the preservation number of CGMCC (China General Microbiological Culture Collection Center) No.20130. The strain has a relatively strong control effect on hemiptera pests such as aphids, aleyrodids, plant hoppers and scale insects, lepidoptera pests such as plutella xylostella, beet armyworms, cabbage caterpillars and prodenia litura, coleoptera pests such as grubs, wireworms and geotrichum candidum, and wax cicada and cockroaches; the compound has an obvious inhibition effect on a plurality of phytopathogens such as damping off, fusarium wilt, black rot, ring rot, red rot, bacterial wilt, leaf spot, sheath blight, smut, root rot, white rot, early blight, gray mold, powdery mildew and anthracnose; by applying the strain fermentation liquor, the plant growth can be obviously promoted; moreover, the method is good in environmental safety and has a good application prospect in the aspect of being developed into biological insecticides and biological pesticides.

Description

technical field [0001] The present invention relates to a kind of Bacillus velesi ZLP-101 and its application. Background technique [0002] Hemipteran pests such as aphids, planthoppers, whiteflies, and scale insects, as well as coleopteran pests such as mites, nematodes, and grubs, bring serious losses to agricultural production and seriously affect the yield and quality of crops. With the continuous expansion of the cultivated area of ​​agricultural protected land and the increase of the multi-cropping index, the air-borne and soil-borne fungal diseases are serious, and multiple diseases often occur together, which has become a serious obstacle to agricultural production. [0003] At present, chemical control is mainly used for diseases. Conventional chemical pesticide control not only kills a large number of natural enemies and beneficial microorganisms, destroys the ecological balance, but also sharply increases the resistance of pests; pesticide residues have caused gr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/22A01P3/00A01P7/04C09K17/14C05F11/08C05G3/60C05G3/80C12R1/07
CPCC12N1/20A01N63/22C09K17/14C05F11/08C05G3/60C05G3/80Y02A50/30
Inventor 张丽萍王雅娜刘洪伟张飞燕董尧坤李文雅王江平刘秋玥赵雯雅
Owner BIOLOGY INST OF HEBEI ACAD OF SCI
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