Gardenia somatic embryogenesis and plant regeneration method

Abstract

The invention is suitable for the technical field of plant cultivation, and provides a cape jasmine somatic embryogenesis and plant regeneration method which comprises the following steps: S1, selecting and pretreating an explant; step S2, induction of the embryonic callus; s3, performing somatic embryo differentiation and multiplication culture; and S4, carrying out somatic embryo germination culture. According to the method, few plant materials are used, the method is not limited by seasons, the propagation coefficient can be maximized within a short time, the seedling raising efficiency can be improved, the production cost can be saved, operation is easy and feasible, repeatability is high, and the method is suitable for annual tests or production; the transplanting survival rate of seedlings is ensured, and the seedling quality is ensured; green production is achieved, the gardenia nursery stock production process is optimized, and the method is worthy of application and popularization.

Description

technical field [0001] The invention belongs to the technical field of plant cultivation, and in particular relates to a gardenia somatic cell embryogenesis and plant regeneration method. Background technique [0002] Gardenia is an evergreen shrub of the Rubiaceae family, and is the first batch of medicinal and food homologous plant resources promulgated by the Ministry of Health. Gardenia leaves, flowers and fruits can be viewed as ornamental, and the flowers and fruits can also be eaten. The active ingredients of the fruits have medicinal value. At present, most of them are wild resources, and man-made damage is very serious, which leads to the serious loss of the excellent germplasm resources of Gardenia, which restricts the healthy development of the gardenia industry. Therefore, an efficient and high-quality resource breeding and conservation approach is sought to protect the excellent germplasm resources of Gardenia. , It is imperative to improve the quantity and qua...

AI Technical Summary

Problems solved by technology

At present, most of them are wild resources, and man-made damage is very serious, resulting in the serious loss of gardenia germplasm resources, which restricts the healthy development of gardenia industry. Therefore, an efficient and high-quality resource breeding and conservation approach is sought to protect gardenia germplasm resources. It is imperative to improve the quantity and quality of gardenia seedlings

[0003] At present, the fast multiplication of Gardenia adopts cutting rapid multiplication and "bud multiplication" tissue culture rapid multiplication mode, but the cutting propagation coefficient is small, the cycle is long, and it is easy to mutate, and the "bud multiplication" tissue culture mode is cumbersome and expensive.

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