Screening method, preparation method and application of deodorization dominant bacteria

Abstract

The invention provides a method for screening and preparing deodorization dominant bacteria and application of the deodorization dominant bacteria. The method specifically comprises the following steps: S1, weighing a sample, and separating and purifying bacterial strains; s2, the deodorization dominant bacteria are screened through primary screening and secondary screening; s3, extracting bacterial DNA and carrying out strain identification; s4, screening out non-antagonistic bacteria by adopting a five-point confrontation method; s5, compounding the microbial inoculants into a complex microbial inoculant, and carrying out orthogonal design; s6, performing ammonia removal and sulfur removal through the strains in different compounding ratios, and recording removal rate data of different compounding ratios to obtain an ammonia removal and sulfur removal ratio of each strain in the complex microbial inoculant; s7, preparing a solid microbial inoculum; the strains in the complex microbial inoculant comprise bacillus thuringiensis, pichia pastoris, bacillus amyloliquefaciens, bacillus licheniformis, bacillus subtillis, actinomycetes and lactobacillus. The bacteria, the saccharomycetes, the bacillus, the actinomycetes and the like for degrading the ammonia gas and the hydrogen sulfide are separated from the kitchen waste biochemical bin sample, the cost is low, the deodorization efficiency is high, and the application range is wide.

Description

technical field [0001] The invention belongs to the technical field of biological deodorization, and in particular relates to a screening, preparation method and application of a deodorant dominant bacteria. Background technique [0002] The types of odor pollutants generated in the process of kitchen waste disposal are complex, and the odor intensity is relatively high. The types of odor substances can be divided into 5 categories according to their composition: sulfur-containing compounds, such as hydrogen sulfide, sulfur dioxide, mercaptans, etc.; nitrogen-containing compounds, Such as ammonia, amines, indole, etc.; halogens and derivatives, such as chlorine, halogenated hydrocarbons, etc.; hydrocarbons and aromatic hydrocarbons; oxygen-containing organics, such as alcohols, phenols, aldehydes, ketones, etc. Gas chromatographic detection showed that the main component of the odor of kitchen waste was ammonia (NH 3 ) and hydrogen sulfide (H 2 S). [0003] The characteri...

AI Technical Summary

Problems solved by technology

[0014] (1) At present, there are few types of bacteria in deodorant bacteria agents, generally only 1-3 species, so the deodorization efficiency is not very high;

[0015] (2) In the compounding of microbial deodorant agents, the appropriate ratio between strains and the antagonism between strains are not fully considered, resulting in unstable product quality and use effects, and related problems emerge in endlessly;

[0016] (3) There is a high-temperature stage in the aerobic composting process of food waste, and the temperature can reach 60-70°C, which will affect the activity of the bacteria in the deodorant agent and ultimately affect the deodorization effect;

[0017] (4) The actual number of viable bacteria in the finished microbial deodorant agent is small and the storage time is short, and the effect of the agent on the actual application scene is not good;

[0018] (5) During the actual use of microbial deodorizing bacteria agents, most people will ignore the cultivation process of the bacteria bed, and will not add the bacteria agent attractant, which is not conducive to the expansion of the bacteria agent cultivation

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