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Wheat wula receptor-like protein kinase gene TuRLK1 and application of Wheat wula receptor-like protein kinase gene TuRLK1

A technology of Urartu wheat and receptor protein, applied in application, genetic engineering, plant gene improvement, etc., can solve problems that are not conducive to the scientific and effective use of resistance genes

Active Publication Date: 2022-07-29
FUJIAN AGRI & FORESTRY UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

These conditions are extremely unfavorable to the scientific and effective use of resistance genes to breed wheat varieties with broad-spectrum and long-lasting resistance

Method used

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  • Wheat wula receptor-like protein kinase gene TuRLK1 and application of Wheat wula receptor-like protein kinase gene TuRLK1
  • Wheat wula receptor-like protein kinase gene TuRLK1 and application of Wheat wula receptor-like protein kinase gene TuRLK1
  • Wheat wula receptor-like protein kinase gene TuRLK1 and application of Wheat wula receptor-like protein kinase gene TuRLK1

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Example 1 Uraltu wheat material PI428309 receptor protein kinase gene TuRLK1 clone of

[0024] Uraltu wheat PI428309 was inoculated with wheat stripe rust after growing to one leaf and one heart stage Pst CY33, inoculated with wheat stripe rust at two time points 0 h and 36 h after inoculation, respectively Pst Uraltu wheat leaves after CY33 were sampled for transcriptome sequencing. Using the Uraltu wheat G1812 genome sequence as a reference, all measured differentially expressed genes in the transcriptome were analyzed. Urartu wheat G1812 receptor-like protein kinase gene TRIUR3 _02522 is a receptor-like protein kinase gene of the LRR-RLK type. Based on the results of transcriptome analysis, inoculation Pst After CY33, TRIUR3 Homologous gene of _02522 in Urartu wheat PI428309 TuRLK1 transcription levels were significantly increased. According to the transcriptome splicing database of Uraltu wheat PI428309, the receptor-like protein kinase gene was pr...

Embodiment 2

[0028] Example 2 TuRLK1 Regulating Urartu wheat PI428309 YrU1 mediated stripe rust resistance

[0029] Using the cDNA of Urartu wheat PI428309 leaf as a template, the primer γ- TuRLK1 -F and γ- TuRLK1 -R is amplified to obtain a PCR product. The Barley stripe mosaicvirus RNAγ (BSMV) plant virus vector was digested with restriction endonuclease Nhe 1 to obtain a linearized plasmid carrying the restriction site. The homologous recombination kit Vazyme ClonExpress® II One Step Cloning Kit C112 was used to The PCR product was constructed into the silencing vector BSMV, and the silencing vector BSMV was obtained: TuRLK1 . With the viral vector BSMV ligated into the GFP fragment: GFP As a control, refer to the method provided in [Holzberg S, Brosio P, Gross C, Pogue GP. 2002. Barley stripe mosaicvirus-induced gene silencing in a monocot plant. Plant J 30: 315-327], infect one leaf and one heart stage Uraltu wheat PI428309, after virus infection, the fourth leaf was take...

Embodiment 3

[0034] Example 3 Common wheat overexpression TuRLK1 Enhances powdery mildew resistance

[0035] See [Shen Q-H, Saijo Y, Mauch S, Biskup C, Bieri S, Keller B, Seki H, Ülker B, Somssich IE, Schulze-Lefert P. 2007. Nuclear Activity of MLA ImmuneReceptors Links Isolate-Specific and Basal Disease-Resistance Responses.Science 315: 1098-1103.] The method provided by single-cell transient expression experiments TuRLK1 The powdery mildew resistance function was verified. Single-cell transient expression mediated by gene gun in the leaves of common wheat Fielder susceptible to powdery mildew TuRLK1 (The gateway system will TuRLK1 The coding region was linked into the intermediate vector pDONR207 and then transferred into the final vector pUBI-GW, amplified TuRLK1 The primers used in the coding region of TuRLK1 -attB-F and TuRLK1 -attB-R), inoculated 4 h after expression Bgt At E09, 48 h later, GUS staining and haustorial index were counted. primer TuRLK1 -attB-F and ...

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Abstract

The invention discloses a Wuraili wheat receptor-like protein kinase gene TuRLK1 and application of the Wuraili wheat receptor-like protein kinase gene TuRLK1. The nucleotide sequence of the Wuraili wheat receptor-like protein kinase gene TuRLK1 is as shown in SEQ ID NO.1, and the amino acid sequence of the protein coded by the Wuraili wheat receptor-like protein kinase gene TuRLK1 is as shown in SEQ ID NO.2. Tests show that the Wuraili wheat receptor-like protein kinase gene TuRLK1 not only is crucial to normal exertion of the resistance function of the wheat stripe rust resistance functional gene YrU1, but also can obviously improve the resistance level of wheat powdery mildew by independently expressing the TuRLK1 in infected wheat; the TuRLK1 is subjected to heterologous expression in arabidopsis thaliana, and the powdery mildew resistance of the arabidopsis thaliana can also be remarkably improved. Therefore, the Wuraili wheat receptor-like protein kinase gene TuRLK1 has relatively strong application potential in plant resistance breeding.

Description

technical field [0001] The invention belongs to the technical field of plant genetic engineering, in particular to a Uraltu wheat receptor protein kinase gene TuRLK And the application of this gene in plant resistance breeding. Background technique [0002] wheat( Triticum aestivum ) is an important food crop related to the national economy and people's livelihood, and diseases such as wheat stripe rust and powdery mildew seriously threaten the growth of wheat and greatly harm the yield and quality of wheat. Wheat stripe rust is caused by the wheat stripe rust fungus ( Puccinia striiformis f. sp. tritici , Pst ) caused by parasitic fungal diseases; wheat powdery mildew is caused by powdery mildew wheat specialized type ( Blumeria graminis f. sp. tritici , Bgt ) caused by parasitic fungal diseases. They can both harm wheat and its relatives, competing with crop leaves for nutrients and hindering photosynthesis. Since the control effect of chemical agents on th...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/54C12N9/12C12N15/82A01H5/00A01H6/46A01H6/20
CPCC12N9/1205C12N15/8282C12Y207/01112Y02A40/146
Inventor 邹声浩汤延胜唐定中
Owner FUJIAN AGRI & FORESTRY UNIV
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