Alkaline xyloglucanase

A technology of xyloglucan and glucanase, applied in the field of alkaline xyloglucanase, can solve the problem of no cellulolytic activity

Inactive Publication Date: 2004-12-01
NOVO NORDISK AS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

True xyloglucanases, however, are true xyloglucan-specific enzymes that catalyze the hydrolysis of xyloglucan to xyloglucan oligosaccharides, but without substantial cellulose Decomposing activity, e.g. activity to hydrolyze the commonly used cellulose-like substrates CMC (carboxymethyl cellulose), HE cellulose and Avicel (microcrystalline cellulose)

Method used

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  • Alkaline xyloglucanase
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  • Alkaline xyloglucanase

Examples

Experimental program
Comparison scheme
Effect test

Embodiment I

[0280] The granular fabric cleaning compositions of the present invention can be prepared as follows:

[0281] Linear C 12 Sodium alkylbenzene sulfonate 6.5

[0282] Sodium sulfate 15.0

[0283] Zeolite A 26.0

[0284] Sodium nitrilotriacetate 5.0

[0285] Enzyme of the present invention 0.1

[0286] PvP 0.5

[0287] TAED 3.0

[0288] Boric acid 4.0

[0289] Perborate 18.0

[0290] Phenolsulfonate 0.1

[0291] minor components up to 100

Embodiment II

[0293] A dense granular fabric cleaning composition (density 800 g / l) according to the invention can be prepared as follows:

[0294] 45AS 8.0

[0295] 25E3S 2.0

[0296] 25E5 3.0

[0297] 25E3 3.0

[0298] TFAA 2.5

[0299] Zeolite A 17.0

[0300] NaSKS-6 12.0

[0301] Citric acid 3.0

[0302] Carbonate 7.0

[0303] MA / AA 5.0

[0304] CMC 0.4

[0305] Enzyme of the present invention 0.1

[0306] TAED 6.0

[0307] Percarbonate 22.0

[0308] EDDS 0.3

[0309] Granular antifoam 3.5

[0310] Water / minor components up to 100%

Embodiment III

[0312] The granular fabric cleaning compositions of the present invention, which are particularly useful in laundering colored fabrics, are prepared as follows:

[0313] LAS 10.7 -

[0314] TAS 2.4 -

[0315] TFAA-4.0

[0316] 45AS 3.1 10.0

[0317] 45E7 4.0 -

[0318] 25E3S - 3.0

[0319] 68E11 1.8 -

[0320] 25E5-8.0

[0321] Citrate 15.0 7.0

[0322] Carbonate - 10

[0323] Citric acid 2.5 3.0

[0324] Zeolite A 32.1 25.0

[0325] NaSKS-6 - 9.0

[0326] MA / AA 5.0 5.0

[0327] DETPMP 0.2 0.8

[0328] Enzyme of the present invention 0.10 0.05

[0329] Silicate 2.5 -

[0330] Sulphate 5.2 3.0

[0331] PvP 0.5 -

[0332] Poly(4-vinylpyridine)-N-oxide - 0.2

[0333] / Vinylimidazole and Vinylpyrrolidine

[0334] Copolymers of ketones

[0335] Perborate 1.0 -

[0336] Phenolsulfonate 0.2 -

[0337] Water / minor components to 100%

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Abstract

A xyloglucanase having a relative xyloglucanase activity of atleast 50% at pH7 and either no or an insignificant cellulolytic activity is obtainable e.g. from a strain of Bacillus. A xyloglucanase comprising an amino acid sequence as shown in positions 30-261 of SEQ ID NO:2 or homologues may be derived from e.g. Bacillus licheniformis, ATCC 14580, and may be encoded by polynucleotide molecules comprising a nucleotide sequence as shown in SEQ ID NO:1 from nucleotide 88 to nucleotide 783; and a xyloglucanase comprising an amino acid sequence as shown in positions 1-537 of SEQ ID NO:4 or homologues may be derived from e.g. B. agaradhaerens, NCIMB 40482, and may be encoded by polynucleotide molecules comprising a nucleotide sequence as shown in SEQ ID NO:3 from nucleotide 1 to nucleotide 1611. The xyloglucanases are useful e.g. in cleaning compositions and for treatment of cellulosic fibres.

Description

[0001] The present invention relates to alkaline xyloglucanases, i.e. enzymes exhibiting xyloglucanase activity as their main enzymatic activity in the neutral and alkaline pH range; to processes for the preparation of these enzymes; also Methods involving the use of these enzymes in the textile, detergent and cellulosic fiber processing industries. Background of the invention [0002] Xyloglucan is a major structural polysaccharide in the primary (growing) cell wall of plants. Structurally, xyloglucan consists of a cellulose-like β-1,4-linked glucose backbone frequently substituted with various side chains. The xyloglucans of most dicots, some monocots and gymnosperms are highly branched polysaccharides in which about 75% of the glucose residues in the backbone bear glycosyl side chains at positions 0-6. The sugar groups directly attached to the branched glucose residues are all α-D-xylose. Since the xylose residue has a β-D-galactose or α-L-fucose-(1-2)-β-D-galactose compo...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/09C11D3/386C12N1/21C12N9/42C12R1/07
CPCC12N9/2434C11D3/38636
Inventor M·舒兰H·奥特鲁普P·L·卓根森M·E·卓恩瓦德
Owner NOVO NORDISK AS
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