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Recombination human cytomegalovirus fusion protein and its preparing method, application

A technology for human cytomegalovirus and fusion protein, which is applied in the field of preparing recombinant human cytomegalovirus fusion protein, and can solve the problems of light color, affecting the formation of antigenic epitopes, and imprecise position of gp52 protein fragments.

Inactive Publication Date: 2005-06-22
李越希 +1
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Problems solved by technology

However, using the fusion protein as an antigen to detect HCMV IgM antibody-positive sera, it was found that the color developed when some sera were detected was lighter, which was presumed to be because the position of the selected gp52 protein fragment was not accurate, which affected the formation of antigenic epitopes.

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  • Recombination human cytomegalovirus fusion protein and its preparing method, application
  • Recombination human cytomegalovirus fusion protein and its preparing method, application
  • Recombination human cytomegalovirus fusion protein and its preparing method, application

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Embodiment Construction

[0106] Detailed description of the embodiment of the present invention:

[0107] 25 Amino Acids of C-terminus of Human Cytomegalovirus PP150 Protein and gp52 Protein

[0108] Fusion expression of C-terminal 164 amino acids

[0109] A gene fragment of 25 amino acids at the C-terminus of the PP150 protein was chemically synthesized by using codons favored by bacteria. The gp52 protein gene fragment was amplified by PCR method. The two gene fragments were cloned into the NcoI / BamHI and BamHI / EcoRI sites in the same plasmid pET28a(+), so that the two were connected in series, and the translation frame was consistent, and a fusion protein could be expressed. The recombinant plasmid was transformed into Escherichia coli BL21 (DE3), and the engineered bacteria that highly expressed the fusion protein was obtained through screening. The expressed fusion protein accounted for about 35% of the total protein in the bacteria and existed in a soluble form.

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Abstract

The recombinant human cytomegalovirus fusion protein and its preparation method and application of the invention relate to the fields of genetic engineering technology and diagnostic reagents. The present invention uses genetic engineering technology to prepare a new type of recombinant protein, which is a fusion protein formed by connecting 25 amino acids at the C-terminal of human cytomegalovirus PP150 protein and 164 amino acids at the C-terminal of gp52 protein. The 25 amino acids at the C-terminal of PP150 protein are fused The N-terminal of the protein, the 164 amino acids of the C-terminal of the gp52 protein are at the C-terminal of the fusion protein, and the two are connected by two amino acids of glycine (Gly) and serine (Ser), and the 162nd amino acid within the 164 amino acids of the C-terminal of the gp52 protein Amino acids were replaced by asparagine (Asn) from lysine (Lys), and 1 methionyl acid (Met) was added to the N-terminal of the fusion protein, with a total length of 192 amino acids. The protein is used for detecting human cytomegalovirus antibody or antigen and for preparing monoclonal antibody and polyclonal antibody.

Description

technical field [0001] The invention utilizes genetic engineering technology to prepare recombinant human cytomegalovirus fusion protein. The two fragments of the fusion protein come from the gp52 protein and PP150 protein of human cytomegalovirus respectively. The two fragments are linked and expressed by genetic engineering technology to form a new fusion protein. The new fusion protein produced can be used as an antigen for human cytomegalovirus. Detection of cell virus antibody or antigen, etc. The invention relates to the fields of genetic engineering technology and diagnostic reagents. technical background [0002] Human cytomegalovirus (HCMV for short) is a DNA virus that can remain latent in the body for a long time after infecting the human body, and can be activated to produce recurrent infection. HCMV-infected pregnant women can not only infect the fetus through the placenta, but also infect the newborn through the birth canal and breast milk. After the fetus an...

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K19/00C12N15/09C12N15/12G01N33/68
Inventor 李越希陶开华
Owner 李越希
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