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Preparation method for tumour cell comprising interleukin-23 gene and its use

A technology of interleukin and tumor cells, which is applied in the field of preparation of tumor cells and can solve the problems of not obtaining clinical curative effect and the like

Inactive Publication Date: 2005-10-26
单保恩
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The treatment of tumors with recombinant cytokines such as interleukin-12, interleukin-2, IFN-α, IFN-γ, and TFN-α has shown certain curative effects, but it is still in the stage of clinical trials and has not yet achieved clinical efficacy the result of

Method used

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  • Preparation method for tumour cell comprising interleukin-23 gene and its use
  • Preparation method for tumour cell comprising interleukin-23 gene and its use
  • Preparation method for tumour cell comprising interleukin-23 gene and its use

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Embodiment Construction

[0038] Embodiments of the present invention are further described below in conjunction with accompanying drawings:

[0039] The overall concept of this embodiment is as follows:

[0040] The vector is retrovirus.

[0041] Using retrovirus as a carrier to introduce interleukin-23 into tumor cells includes the following steps:

[0042] (1) Using guanidine isothiohydrogen one-step method to extract tissue total RNA from mouse splenocytes;

[0043] (2) Obtain mouse interleukin-23p19 and p40 cDNA by reverse transcription polymerase chain reaction method, insert the obtained cDNA into the Pcr2.1 carrier in the TA Cloning kit, perform sequencing with a DNA sequencer after parallel polymerase chain reaction Verify the correctness of the cDNA; insert the cDNA after the verified sequence into the viral vector with restriction endonuclease and DNA ligase;

[0044] (3) using a kit (product of Invitrogen, USA) to introduce the viral vector obtained in step (2) into a packaging cell, whi...

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Abstract

The present invention belongs to the preparation process of transgenic tumor cell for treating malignant tumor, and is especially the preparation process of tumor cell containing interleukin-23 transgene. The tumor treating method of the present invention is superior to available tumor treating methods. Interleukin-23 gene is introduced via carrier into tumor cell to produce interleukin-23 with powerful tumor resisting effect to treat malignant tumor. It functions in the initial immune response stage and activate dendritic cell to induce natural killing of cell. Interleukin-23 can also induce the proliferation and activation of memory T cell, secret IFN-gamma, regulate immunological function, inhibit the vascularization in tumor part and thus reach the effect of curing malignant tumor.

Description

Technical field [0001] The invention belongs to a method for preparing transgenic tumor cells for treating malignant tumors, in particular to a method for preparing interleukin-23 gene-transferred tumor cells. Background technique [0002] At present, the main treatment methods for tumors include surgical resection, radiotherapy and chemotherapy. These treatments have certain side effects on the body and do not have the effect of improving the prognosis in the long term. For example, chemotherapy and radiation therapy can often inhibit bone marrow hematopoiesis and the body. Immunological function, leading to susceptibility to infection and low anti-tumor immune response; surgical trauma can also suppress various immune functions of the body, and there is a risk of expanding micrometastases. The direct use of cytokines to treat tumors has achieved certain curative effects. However, the half-life of cytokines in the body is short, and it is difficult to m...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K48/00A61P35/00C12N5/00C12N15/86C12N15/88
Inventor 田川雅敏单保恩
Owner 单保恩
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