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Novel usage of soluble P-lectin and anthrax lethal toxin

A technology of toxin and anthrax, applied in the field of soluble platelet-selectin, can solve the problem of not mentioning the treatment of systemic bleeding diseases and other problems, and achieve no adverse side effects, good effect, and the effect of promoting blood coagulation

Inactive Publication Date: 2007-07-04
张新侯
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, these documents also do not mention any recommendations for the treatment of systemic bleeding disorders

Method used

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  • Novel usage of soluble P-lectin and anthrax lethal toxin
  • Novel usage of soluble P-lectin and anthrax lethal toxin
  • Novel usage of soluble P-lectin and anthrax lethal toxin

Examples

Experimental program
Comparison scheme
Effect test

example 1

[0044] Example 1 Plasma agglutination test of hemorrhagic dengue fever and anthrax toxin

[0045] In the plasma agglutination test, the blood of mouse C57BL / 6J was centrifuged at 1,500×g for 25 minutes to obtain “platelet-poor plasma (PPP)” of the mouse. The resulting plasma was added to an equal volume of 20 millimolar calcium chloride (CaCl 2 ), at 800rpm and 37°C, the changes in absorbance due to agglutination were recorded with an aggregometer (aggregometer: model 600B, Ion-Trace, Stouffville, Canada) and the plasma clotting time was measured.

[0046] In this example, the following reagents were used for plasma agglutination test: recombinant P-selectin-Ig Fc protein (purchased from R & D Systems Inc., Minneapolis, Minnesota, USA) as the source of P-selectin. The dengue virus protein uses the first non-structural protein of dengue virus, which is derived from the first non-structural protein gene ( Chang et al., 2002, J Infect Dis 186, 743-51). The anthrax toxin uses r...

example 2

[0049] Example 2 Mice protection test of anthrax toxin and hemorrhagic snake venom

[0050] In the protective power test of P-selectin on mice, the mice were first administered with recombinant P-selectin-Ig protein. Four hours after P-selectin was added with neutralizing monoclonal antibody or human immunoglobulin IgG (both at a dose of 1.2 g / g), anthrax toxin and hemorrhagic snake venom were administered intravenously. Then the death time of the mice was observed. The sources of recombinant P-selectin-Ig protein and anthrax toxin are shown in Example 1. The hemorrhagic snake venoms used the venoms of western diamondback rattlesnake (Crotalus atrox) and eastern diamondback rattlesnake (Crotalus adamanteus) (purchased from Sigma, St. Louis, Missouri, USA).

[0051] As shown in FIG. 2A , after administering the venom of the western diamondback rattlesnake (Crotalus atrox) and the eastern diamondback rattlesnake (Crotalus adamanteus), all six mice (C57BL / 6J) died within 1 hour...

example 3

[0053] Mice protection test of example 3 bacteremia

[0054] The reagents and methods of the mouse protection test are shown in Example 2. Bacteremia in mice was injected into the plasma of mice (C57BL / 6J) to simulate bacteremia with 1×108 bacteria count / gram of Escherichia coli liquid. As shown in Table 1, all 6 mice injected with the E. coli solution died within 24 hours. However, mice given prior recombinant soluble P-selectin survived an average of 2 days longer. Therefore, soluble P-selectin does have a protective effect on bacteremic mice.

[0055] Mice Handling

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Abstract

The invention relates to the use of soluble P-lectine in preparing medicament for treating whole body haemorrhage diseases, and the use of an anthracnose lethiferous toxin in preparing medicament for treating antithrombotic diseases.

Description

technical field [0001] The present invention relates to a novel use of soluble platelet-selectin (P-selectin, platelet-selectin, P-selectin) for treating systemic bleeding diseases. Background technique [0002] The types of bleeding disorders are very diverse and their etiology is quite complicated, such as abnormal structure of blood vessel wall or connective tissue, abnormal platelet quantity or function, abnormal coagulation factors, excessive fibrinolysis and circulating anticoagulant substances, etc. Bleeding disorders can be classified into hereditary and non-hereditary bleeding disorders based on whether they are inherited or not. Inherited bleeding disorders such as hemophilia are caused by mutations in blood coagulation factors that result in the loss or reduction of blood coagulation ability. In clinical cases of bleeding, most of them are non-hereditary bleeding diseases, including bleeding caused by internal and external trauma, bleeding caused by insufficient ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K38/02A61K45/00A61P7/04A61P9/10
CPCY02A50/30
Inventor 张新侯孙德珊
Owner 张新侯
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