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Cosmetic compositions

A technology of composition and skin care composition, applied in the direction of cosmetics, drug combinations, cosmetic preparations, etc., can solve the problems of no disclosure of resveratrol skin care use or beauty use, no mention of resveratrol, etc.

Inactive Publication Date: 2001-12-19
UNILEVER NV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0011] The above-mentioned prior art does not disclose the skin care use or cosmetic use of resveratrol, does not mention that resveratrol is a kind of phytoestrogen, or it can inhibit keratinocyte proliferation, or it can promote keratinocyte differentiation, or it May regulate skin irritation caused by alpha hydroxy acids

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] This example illustrates that resveratrol is a phytoestrogen.

[0047] The following assay was used to determine whether resveratrol had estrogen-like activity.

[0048] The ZR75 cell line is a ductal breast cancer cell line originally isolated from the malignant breast epithelium of a 36-year-old Caucasian woman (Engel et al., Human Breast Cancer Cells in Continuous Culture: A Review, Cancer Res. , 38:4327-4339, 1978). This cell line contains receptors for estrogen, progesterone, and other steroid hormones, but responds only to estrogen with enhanced proliferation. This cell line contains high-affinity estrogen-specific receptors. Therefore, this cell line is suitable for testing estrogen-like activity (Markiewicz et al., "In vivo bioassay of non-steroidal phytoestrogens", Journal of Biochemistry, Molecular and Biological Steroids (J. Steroid Biochem. Molec. Biol.), 45:399-405, 1993). The method for measuring the rate of DNA synthesis in cells:

[0049] Will 3 H-...

Embodiment 2

[0057] This example demonstrates that resveratrol can inhibit the proliferation of keratinocytes. 1. Let trypsinized normal human keratinocytes isolated from neonatal foreskin grow in Dulbecco's modified Eagle (DME) medium / 5% fetal bovine serum in the presence of mitomycin C-treated 3T3 mouse fibroblasts designed to establish dividing keratinocyte populations. Keratinocytes were grown under the above conditions until their third passage appeared. 2. For testing, third passage keratinocytes were plated and the plates were placed in serum-free Keratinocyte Growth Medium (KGM - from Clonetics, San Diego, CA) containing 0.09 mM calcium. Approximately 30 000 cells were added to each well of a 6-well cell culture plate and allowed to grow for 5 days until the cells reached approximately 40% confluency. 3. The medium was refreshed (KBM - from Clonetics) and different concentrations of resveratrol (as indicated in Table 2) were added to the medium from a DMSO (dimethyl sulfoxide) st...

Embodiment 3

[0064] Example 2 was repeated with other different concentrations of resveratrol. The results obtained are summarized in Table 3.

[0065] table 3

[0066] Resveratrol Concentration

[0067] Find out from the result of table 3, when resveratrol concentration is lower than 0.8 μ M (or 0.000018% (weight)), wherein include very low concentration 0.33 μ M (this concentration is the resveratrol in 0.5% grape extract disclosed by JP6336421 The maximum concentration of retrol) could not effectively reduce the proliferation of keratinocytes.

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PUM

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Abstract

Resveratrol, a component found in common edible plants, including peanuts and red grapes, is a phytoestrogen. Resveratrol can inhibit the proliferation of skin epidermal cells (keratinocytes) and promote their differentiation. Resveratrol has also been found to relieve skin irritation caused by alpha hydroxy acids. Resveratrol is effective in improving the appearance of wrinkled, lined, dry, flaky, aged or photodamaged skin and improving skin thickness, elasticity, flexibility, radiance, radiance and fullness.

Description

field of invention [0001] The present invention relates to cosmetic compositions containing resveratrol, a natural plant-derived estrogen, and cosmetic methods for conditioning the skin by applying the composition to the skin. Background of the invention [0002] Human skin is mainly composed of two layers, the thicker bottom layer is the dermis, and the thinner top layer is the epidermis. The dermis provides strength, elasticity and thickness to the skin. With aging, the thickness of the dermis decreases, and it is certain that wrinkle formation in aging skin is partly responsible for this. The top layer of human skin, or epidermis, that gives skin its resilience and barrier properties is made up of many different types of cells. Keratinocytes are the major type of epidermal cells (accounting for 75-80% of the total number of human epidermal cells). In the epidermis, keratinocytes exist in four distinct stages of differentiation. Epidermal differentiation is important t...

Claims

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Application Information

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IPC IPC(8): A61K8/00A61K8/25A61K8/30A61K8/33A61K8/34A61K8/36A61K8/365A61K8/37A61K8/41A61K8/44A61K8/60A61K8/63A61K8/67A61K8/72A61K8/892A61K8/92A61K8/96A61K8/99A61Q17/04A61Q19/00A61Q19/02A61Q19/08
CPCA61K8/347A61Q17/04A61Q19/00A61Q19/004A61Q19/007A61Q19/02A61Q19/08A61P17/00A61K8/34
Inventor R·G·卡森K·帕特尔M·巴锐特C·A·波斯科S·皮莱
Owner UNILEVER NV
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