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Stem and ancestral cell suspension for embryo hematogenesis and its preparing method and use

A cell suspension, progenitor cell technology, applied in embryonic cells, biochemical equipment and methods, germ cells, etc., can solve the problem of low success rate, difficult to meet the quantitative requirements of overdose, hematopoietic stem and progenitor cells, etc. problem, to achieve the effect of low cost

Inactive Publication Date: 2002-08-21
黄斌 +2
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AI Technical Summary

Problems solved by technology

Extracting stem and progenitor cells from embryonic fetal liver for clinical application. From the 1980s to the 1980s, domestic and foreign scholars made separate attempts. They all obtained hematopoietic stem and progenitor cells from fetal fetal liver at 16-22 weeks for clinical use. Cell transplantation, but the total number of hematopoietic stem and progenitor cells in this period is relatively small, and it is difficult to meet the requirement of overdose; in addition, due to developmental barriers, it is difficult for hematopoietic stem and progenitor cells in the allogeneic fetal liver to be transferred to the recipient's bone marrow Homing, because the microenvironment is quite different, so the success rate is low

Method used

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Examples

Experimental program
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Effect test

Embodiment 2

[0116] Embodiment two: fetal weight 1601kg (fetal liver weight 70g, spleen weight about 5.4g)

[0117] The stillborn fetus with a gestational age of more than 22 weeks induced by water bag was delivered and stored at 10°C, and sent to the laboratory within 3 hours. Under sterile conditions and at room temperature of 20°C, fetal blood samples were first extracted from the fetal heart for anticoagulation. The blood type and HLA antigen were tested, and then the relevant fetal bones and livers were dissected out, placed in culture medium, and stored at 4°C.

[0118] A. Separation and preparation of fetal bone marrow hematopoietic stem and progenitor cell suspension:

[0119] (1) Use bone forceps, bone scissors and scalpel scissors to remove the periosteum and cartilage at both ends of the fetal bone;

[0120](2) Insert the needles into the osteoporotic matter at both ends with a culture fluid syringe with anticoagulant in it, and pressurize the bone marrow into the collection bo...

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Abstract

The present invention relates to the modern biological technological process of preparing hemotopoietic stem and ancestral cell suspension for clinical hematopoietic stem and ancestral cell transplantation and treatment of relative diseases. The said biochip product is prepared from donative dead fetus over 22 weeds age through separation and extraction and consists of two parts of fetus marrow hemoatopoietic stem and ancestrall cell suspension and fetus liver hematopoietic stem and ancestral cell suspension. The preparation includes the steps of low temperature maintenance of dead fetus at 0-10 deg.c for 1-16 hr, drawing blood for relevant detection, dissection to take fetus bone and fetus liver, maintenance and culture liquid for 1-12 hr, separation and extraction to obtain the hematopoietic stem and ancestral cell suspension for clinical application directly or after being freeze dried.

Description

technical field [0001] The embryonic hematopoietic stem and progenitor cell suspension and its preparation method and application of the present invention relate to the use of modern biotechnology to separate and extract high-quality and super-dose hematopoietic stem and progenitor cells from embryos and the method thereof, which can be applied to clinical hematopoietic stem and progenitor cells Transplantation, a biological product for the treatment of related blood diseases, genetic disease tumors, and immune function damage caused by radiotherapy and chemotherapy. Background technique [0002] Clinical hematopoietic stem and progenitor cell transplantation began with adult bone marrow transplantation. Bone marrow is extracted from the donor bone marrow by surgery, which causes trauma to the donor that is unbearable for ordinary people. In addition, the hematopoietic stem and progenitor cell antigens in the adult bone marrow are fully expressed, and the differentiation and...

Claims

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Application Information

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IPC IPC(8): A61B17/00A61K35/545C12N5/0735C12Q1/02
Inventor 黄斌黄宁黄裕权付中苓潘欣彭蕾孙俊
Owner 黄斌
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