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High metastasis human breast cancer cell system and its establishing method

A high-metastasis technology of human breast cancer cells, applied in the field of cancer cell lines, human breast cancer cell lines and their establishment, can solve the problems of inaccurately reflecting the clinical process of tumor growth and metastasis, affecting the in-depth study of metastasis mechanism intervention therapy, etc.

Inactive Publication Date: 2004-02-11
NANKAI UNIV
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Problems solved by technology

However, current studies on tumor metastasis are mainly limited to surgically resected tumor specimens and the observation of metastasis-related indicators of certain tumor cell lines with limited metastatic characteristics, and none of them can accurately reflect the entire clinical process of tumor growth and metastasis, directly affecting In-depth research on the mechanism of metastasis and its intervention therapy

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  • High metastasis human breast cancer cell system and its establishing method
  • High metastasis human breast cancer cell system and its establishing method
  • High metastasis human breast cancer cell system and its establishing method

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Embodiment Construction

[0018] The concrete method of the human breast cancer cell line that the present invention establishes is as follows:

[0019] 1) Inoculation of SCID mice: Take breast cancer parental MCF-7 cell suspension (5×10 7 / 0.2ml) was subcutaneously inoculated on the shoulder of SCID mice to establish a SCID mouse breast cancer metastasis model. SCID mice were provided by Tianjin Institute of Hematology, Chinese Academy of Medical Sciences.

[0020] 2) Confirmation of metastases: the SCID mice were killed, the pathological sections of each tissue were observed under a microscope, and the metastases were determined to be lung, spleen, bone marrow, and lymph nodes.

[0021] 3) Obtaining a human breast cancer cell line with high metastasis tendency: 68 days after inoculation of MCF-7 cells, the SCID mice were sacrificed, and the lungs were taken out for primary culture. Use the following steps: rinse the tissue block with Hank’s solution of double antibody for 5 minutes; cut the tissue ...

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Abstract

The present invention relates to cancer cell system. The establishment of tumor metastasis animal model is essential for the deep research of tumor invasion and metastasis mechanism. SCID mouse is used as experiment target, and the establishment includes subcutaneously inoculating breast cancer parent MCF-7 cell suspension in shoulder of SCID mouse, killing SCID mouse 68 days after inoculation to take out lung for primary culture, cell passage after cells fill the bottom of the culture bottle with the cell being named as LM-MCF-7, subcutaneously re-injecting tumor cell to SCID mouse after 20 generation culture in vivo of LM-MCF-7 and repeating the said steps. The process has tumor forming rate of 100 % and wide metastasis in lung, kidney, spleen, marrow, lymph node, heart, etc. of SCIDmouse is found.

Description

technical field [0001] The invention relates to a cancer cell line, in particular to a human breast cancer cell line with a high metastasis tendency and a method for its establishment, belonging to the field of animal cell lines. Background technique [0002] Tumor invasion and metastasis are one of the most basic biological characteristics of malignant tumors, and they are also an important reason for the death of tumor patients. However, the exact mechanism of transfer is not well understood. Therefore, tumor metastasis has always been a hot spot in oncology research. However, current studies on tumor metastasis are mainly limited to surgically resected tumor specimens and the observation of metastasis-related indicators of certain tumor cell lines with limited metastatic characteristics, and none of them can accurately reflect the entire clinical process of tumor growth and metastasis, directly affecting In-depth research on the mechanism of metastasis and its intervent...

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Application Information

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IPC IPC(8): C12N5/00
Inventor 叶丽虹张晓东朱惠芳吴莲英鞠永健
Owner NANKAI UNIV
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