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Method for producing ramification LYCD of living yeast

A production method and yeast cell technology, applied in biochemical equipment and methods, microorganisms, treatment of microorganisms with electricity/wave energy, etc., can solve problems such as health damage, achieve high benefits, low production costs, and easy promotion

Inactive Publication Date: 2004-03-10
TIANJIN UNIVERSITY OF SCIENCE AND TECHNOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] In view of the above situation, the present invention solves the problem of health damage caused by various extreme environments, and provides a new production method, which utilizes various extreme environments to stimulate yeast cells to change its metabolic mechanism to produce protective properties against different stimulation methods. Substances, combined with modern biotechnology, extract them as bioactive additives

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] Utilize the extreme oxidative environment in which live yeast survives, that is, use substances with high redox potential, such as H 2 o 2A method for stimulating, producing live yeast cell derivatives, comprising the steps of:

[0043] ●Select yeast as the source of bacteria.

[0044] ●Cultivation medium: Wort solid medium is used for slant preservation, and medium containing peptone, glucose, yeast extract and inorganic salts suitable for yeast growth is used as liquid medium. All slant strains were cultured statically for 48 hours at 28-30°C.

[0045] ●Cultivation of strains: slant strains → liquid strains → liquid culture to logarithmic phase.

[0046] H 2 o 2 Stimulation: add H to the culture medium 2 o 2 , H 2 o 2 Concentration: 0.1mM, 28-30°C water bath shaker culture ●Collection of bacteria: H 2 o 2 The stimulated culture solution was collected by centrifugation.

[0047] ●Bacterial wall breaking: use The liquid nitrogen method disrupts the cell wal...

Embodiment 2

[0051] The extreme oxygen environment for living yeast to survive is H 2 o 2 , a method to stimulate the production of live yeast cell derivatives: adding H to the culture medium 2 o 2 , H 2 o 2 The concentration is 0.5mM, and cultured on a shaker in a water bath at 28-30°C for 3h. Other steps are the same as

[0052] Example 1.

Embodiment 3

[0054] The extreme oxygen environment for living yeast to survive is H 2 o 2 , a method to stimulate the production of live yeast cell derivatives: adding H to the culture medium 2 o 2 , H 2 o 2 The concentration is 1mM, and cultured on a water bath shaker at 28-30°C for 0.5h. Other steps are the same as

[0055] Example 1.

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PUM

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Abstract

A process for preparing the living yeast cell derivative LYCD used as the additive of functional food, functional cosmetics and medicines features that different extreme environments are used to respectively stimulate the yeast cells for changing its metabolism mechanism and generating said LYCD, which is extracted by modern biologic wall-breaking technique. Said LYCD has high resistance to oxidizing, sanility, high temp. low temp. ionic radiation, electromagnetic radiation, acid alkali, etc.

Description

Technical field: [0001] The invention belongs to the technical field of bioactive additive production, and in particular relates to a method for producing living yeast cell derivatives by using various extreme environmental stimuli. technical background: [0002] With the development of the economy and the continuous improvement of people's living standards, people have higher requirements for health, and they are increasingly aware that there are many factors that are unfavorable to health in the living environment and working environment. In order to effectively prevent adverse external environments from harming the body and enhance the internal resistance of the body, functional cosmetics with anti-oxidation, anti-aging, heat resistance, cold resistance, ionizing radiation resistance, electromagnetic radiation resistance, osmotic pressure resistance, acid and alkali resistance, Food and pharmaceutical products, and these products are more and more popular. Therefore havi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23L33/145C12N1/16C12N1/38C12N13/00C12P1/02
Inventor 路福平杨华杜连祥戚薇
Owner TIANJIN UNIVERSITY OF SCIENCE AND TECHNOLOGY
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