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Polygonatum cyrtonema Hua regeneration system establishment method

A construction method, the technology of Polygonatum, applied in the field of establishment of Polygonatum plant regeneration system, can solve the problems of etiolation, vitrification, callus produced by explants, etc., and achieve the effect of fast growth

Inactive Publication Date: 2004-12-29
SICHUAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

For Rhizoma Polygonatum, it is prone to yellowing or vitrification in tissue culture. So far, it has not been seen to induce its explants to produce callus, and then form a regeneration system through tissue differentiation, and in this Discovery of mannose-binding lectins in rhizomes of regeneration system

Method used

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  • Polygonatum cyrtonema Hua regeneration system establishment method
  • Polygonatum cyrtonema Hua regeneration system establishment method
  • Polygonatum cyrtonema Hua regeneration system establishment method

Examples

Experimental program
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Effect test

Embodiment 1

[0032] 1. Plant material

[0033] Polygonatum cyrtonema Hua (Polygonatum cyrtonema Hua) is Polygonatum cyrtonema Hua of the genus Polygonatum cyrtonema in the family Liliaceae. The materials were taken from the mountainous area of ​​Changning County, Sichuan Province.

[0034] 2. Callus induction medium

[0035] The culture medium consists of:

[0036] (1) MS basal medium consists of: macroelements (1.65g / L NH 4 NO 3 , 1.9g / L KNO 3 , 0.44g / L CaCl 2 .2H 2 O, 0.37g / L MgSO 4 .7H 2 O, 0.17g / L KH 2 PO 4 ), trace elements (37.25mg / L Na 2 EDTA, 27.8mg / L FeSO 4 .7H 2 O, 0.83mg / LKI, 6.2mg / LH 3 BO 3 , 22.3mg / L MnSO 4 .4H 2 O, 8.6mg / L ZnSO 4 .7H 2 O, 0.25mg / L Na 2 MoO 4 .2H 2 O, 0.025mg / L CuSO 4 .5H 2 O, 0.025mg / L CoCl 2 .6H 2 O), 2mg / L glucosinolate, 100mg / L inositol, 0.1mg / L thiamine hydrochloride (B1), 0.5mg / L pyridoxine hydrochloride (B6), 0.5mg / L niacin.

[0037] (2) Agar 8g / L, sucrose 30g / L.

[0038] (3) Hormone composition and content are shown in Table...

Embodiment 2

[0059] 1. Materials

[0060] The callus of Cyst Polygonatum callus induced by Example 1.

[0061] 2. Medium

[0062] The callus differentiation medium is: MS basic medium, 0.50mg / L 2,4-D+0.5mg / L6-BA, 8g / L agar, 30g / L sucrose, pH5.8.

[0063] 3. Culture conditions

[0064] After 6 weeks, the callus grown in Example 1 was directly transferred into the differentiation medium, and cultured for 2-3 weeks at 25±2° C., 13 h / d light, and 2000 Lx light intensity.

Embodiment 3

[0066] The medium is: MS basic medium, 1.0mg / L 2,4-D, A1.0mg / L 6-B, 8g / L agar, 30g / L sucrose, pH5.8.

[0067] The culture conditions are as follows: the temperature is 25±2°C, the light is 14h / d, and the light intensity is 1800Lx.

[0068] Others are identical with embodiment 2.

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Abstract

The establishing process of Polygonatum cyrtonema Hua regenerating system includes the following steps: inducing the callus of Polygonatum cyrtonema Hua explant; inoculating the callus to differential culture medium and inducing seedling growth; shifting the un-rooted seedling to rooting culture medium to induce rooting; hardening off and transplanting. By means of the process of the present invention, Polygonatum cyrtonema Hua regenerating system may be obtained in 3-4 months and the transplantation survival rate reaches 70-75 %. Compared with medicinal sealwort, the rhizome has similar content of mannose combined hemoglutinin and blood coagulating activity.

Description

technical field [0001] The invention relates to a method for establishing a regeneration system of Polygonatum genus with medicinal value, in particular to a method for establishing a regeneration system for Polygonatum cystatidis. Background technique [0002] Polygonatum sibiricum Redoute, Polygonatum cyrtonema Hua, Polygonatum macropodium Turcz., Polygonatum kingianum Coll.et Hemsl., Polygonatum cirrhifolium (Wall .) Royle) etc. rhizome [1] . Polygonatum has been used as medicine to treat diseases in ancient times. "Shen Nong's Materia Medica" listed it as the top grade. Polygonatum is sweet in nature and flat in taste, and belongs to the lungs, spleen, kidneys, and meridians. It is a commonly used medicine in traditional Chinese medicine to nourish and strengthen the body and treat kidney deficiency, dry cough due to lung deficiency, and spleen and stomach deficiency. Modern pharmacological research proves that Polygonatum has various pharmacological effects such as e...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 鲍锦库吴传芳安洁高顺赵曦常丽青荣艳珍吕鸿周何小佳洪志霞邓洁刘超顾莹陈放
Owner SICHUAN UNIV
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