Biochemical detection liquid for glucose in urine
A technology for biochemical detection and urine sugar, applied in biological testing, analysis through chemical reactions of materials, material inspection products, etc., can solve problems that have not been done before, and achieve the effect of strong versatility and social popularization
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Embodiment 1
[0026] Preparation of urine glucose test solution
[0027] Process requirements:
[0028] Trisodium citrate (sodium citrate) plus anhydrous sodium carbonate is first heated with a small amount of water to help dissolve, then copper sulfate is added and stirred until it is completely dissolved, and distilled water is added to the total amount required. If there are impurities, filter and bottle for inspection .
[0029] The obtained product is a blue solution with a pH of 10.7±0.2 and an absorbance of 0.15-0.32A at 430 nm, and is stored in a sealed white bottle at room temperature.
Embodiment 2
[0031] Sensitivity of urine sugar biochemical detection solution
[0032] Weigh 4 grams of glucose dry powder, dissolve in 100ml of distilled water, and prepare a 4w / v% glucose solution. According to 2-fold serial dilution, prepared as 2w / v%, 1w / v%, 0.5w / v%, 0.25w / v%, 0.125w / v%, 0.0625w / v%, 0.03w / v%, 0.015w / v% simulated diabetic urine, and indicate the concentration.
[0033] g%
[0034] The above experiments show that the sensitivity of the detection solution of the present invention can reach about 0.03-0.015%.
Embodiment 3
[0036] Glucose metabolism detection
[0037] g%
[0038] The urine sugar concentration range suggested by the above color development results is completely consistent with the concentration range detected by the classical method.
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