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Free radicle production method and uses of lactochrome and its derivative composition dark reaction

A technology of riboflavin and derivatives, applied in the application field of China, to achieve the effect of inhibiting the growth of fungi

Inactive Publication Date: 2005-04-13
山东省生物信息工程技术研究中心 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Previous studies have shown that riboflavin can generate reactive oxygen species under light conditions, and this phenomenon can be used to remove environmental pollutants (Pajares et al, Journal of Photochemistry and Photobiology A: Chemistry, 139: 199-204, 2001), However, there is no report on the generation of free radicals by the dark reaction of riboflavin

Method used

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  • Free radicle production method and uses of lactochrome and its derivative composition dark reaction
  • Free radicle production method and uses of lactochrome and its derivative composition dark reaction

Examples

Experimental program
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Effect test

Embodiment 1

[0035] Example 1 Preparation of Riboflavin Dark Reaction Free Radical Generating Composition

[0036] The composition comprises riboflavin (obtained by chemical synthesis or fermentation), surfactant micelles (Triton X-100 is selected) and a reducing agent with low redox potential (potato juice is selected).

[0037] Its mass composition is: riboflavin: surfactant micelles: reducing agent=1: 21.63: 18.5 of low redox potential

[0038] (Note: For the sake of brevity, the abbreviations are used instead in the following descriptions. Riboflavin and its derivatives are referred to as "riboflavin"; surfactant micellar solutions are referred to as "micelle"; reducing agents with low redox potential are referred to as "reducing agent".)

[0039] operate:

[0040] Riboflavin micellar solution preparation

[0041] Take 1.264 g of surfactant Triton X-100, dissolve it in 200 ml of phosphate buffer, stir gently to avoid foaming, and dilute to 250 ml for later use. Weigh 0.058g of ribo...

Embodiment 2

[0059] Example 2 Preparation of Riboflavin Dark Reaction Free Radical Generating Composition

[0060] The composition comprises riboflavin (obtained by chemical synthesis or fermentation, the same below), micelles (select Tween-20 in polyoxyethylene sorbitan fatty acid ester) and reducing agent (select cysteine ). Its mass composition is:

[0061] Riboflavin: micelles: reducing agent=1: 50: 20; the rest are the same as in Example 1.

Embodiment 3

[0062] Example 3 Preparation of Riboflavin Dark Reaction Free Radical Generating Composition

[0063] The composition comprises riboflavin, micelles (tween-40 is selected) and reducing agent (cysteine ​​is selected). Its mass composition is: riboflavin: micelles: reducing agent = 1: 200: 2. All the other are with embodiment 1.

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Abstract

The present invention is the method of utilizing the dark reaction of riboflavin and its derivative and its application. Riboflavin and its derivative is dispersed in the surfactant micelle solution and mixed with reductant with lower redox potential to obtain free radical generating dark reaction composition. The composition has the mass composition of riboflavin and its derivative to surfactant micelle solution to reductant with lower redox potential of 1 to 10-3000 to 0.1-20. Under dark condition, the composition can generate great amount of free radicals to suppress the growth of fungi effectively with less resistance. The present invention may be used as medicine for preventing and treating microbe caused diseases and diseases of crops, and may be also used in preparing bactericide and preparation for degrading dark environment pollutant. The present invention lays foundation of the wide application of riboflavin and its derivative.

Description

technical field [0001] The invention belongs to the field of free radical chemistry, and in particular relates to a method for generating free radicals by dark reaction of riboflavin and its derivatives, and its application in fungicides and preparations for degrading environmental pollutants. Background technique [0002] Riboflavin (Riboflavin) also known as vitamin B 2 , vitamin B2, flavin (Lactoflavin), ovoflavin (Ovoflavin), vitamin G (Vitamin G), vitamin G; riboflavin is widely distributed in all leafy vegetables, warm-blooded animals and fish meat; for orange Yellow crystalline powder, slightly odorous, slightly bitter taste; it is stable at normal humidity and not affected by oxygen in the air and diffuse light. Stable in neutral or acidic solutions: unstable under alkaline conditions or when exposed to visible or ultraviolet light; virtually insoluble in water, ethanol, chloroform, or ether; in hot water, surfactant micellar solutions, or dilute hydrogen dissolved...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N43/90
Inventor 张红雨孙振令
Owner 山东省生物信息工程技术研究中心
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