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Novel cell and its use for preparing gastrodin

A technology of gastrodin and cells, which is applied in the field of new cells and its use in the preparation of gastrodin, and can solve the problems of poor economic benefits, high preparation costs, and easy pollution of the environment

Active Publication Date: 2005-10-05
KPC PHARM INC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the existing synthetic gastrodin process has defects such as high preparation cost, flammable raw materials, easily polluted environment and poor economic benefits.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0012] Embodiment 1. The preparation of gastrodin

[0013] Add 100ml of LS liquid medium and 15g of cells (strain) (CGMCC No.1122) into a 250ml Erlenmeyer flask, then add 70.00mg of p-Hydroxybenzaldehyde and culture in a shaker at room temperature for 12 days with a rotation speed of 100-120 rpm. The conversion solution was extracted with n-butanol, then the n-butanol extract was concentrated, and the concentrated residue was chromatographed on a silica gel column, eluted with chloroform:methanol (9:1, v / v), concentrated and eluted liquid to obtain crude gastrodin, which was recrystallized from chloroform:methanol (9:1, v / v) to obtain 56.66 mg of gastrodin with a yield of 80.95% and a melting point of 154-156°C.

Embodiment 2

[0014] Embodiment 2. The preparation of gastrodin

[0015] Add 100ml LS liquid medium and 15g cells (strains) (CGMCC No.1122) to a 250ml Erlenmeyer flask, rotate in a shaker at room temperature for 4-5 days at a speed of 100-120 rpm, and then add 30.00mg of Hydroxybenzaldehyde was cultured on a shaker at room temperature for 10 days with a rotating speed of 100-120 rpm. The conversion solution was extracted with n-butanol, then the n-butanol extract was concentrated, and the concentrated residue was chromatographed on a silica gel column, eluted with chloroform:methanol (9:1, v / v), concentrated and eluted liquid to obtain crude gastrodin, which was recrystallized from chloroform:methanol (9:1, v / v) to obtain 24.89 mg of gastrodin with a yield of 82.96% and a melting point of 154-156°C.

Embodiment 3

[0016] Embodiment 3. The preparation of gastrodin

[0017] Add 100ml LS liquid medium and 10g cells (strains) (CGMCC No.1123) to a 250ml Erlenmeyer flask, rotate in a shaker at room temperature for 4-5 days at a speed of 100-120 rpm, and then add 30.00mg of Hydroxybenzaldehyde was cultured on a shaker at room temperature for 10 days with a rotating speed of 100-120 rpm. The conversion solution was extracted with n-butanol, then the n-butanol extract was concentrated, and the concentrated residue was chromatographed on a silica gel column, eluted with chloroform:methanol (9:1, v / v), concentrated and eluted liquid to obtain crude gastrodin, which was recrystallized from chloroform:methanol (9:1, v / v) to obtain 25.80 mg of gastrodin with a yield of 86.00% and a melting point of 154-156°C.

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PUM

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Abstract

This invention relates to new cell from plants and its utilization in gastrodia tuber element and the preparation method. The plant cells of this invention are from Datura, especially the white and violet flower stramonium.

Description

field of invention [0001] The present invention relates to a novel plant-derived cell, its use in preparing gastrodin and a method for preparing gastrodin. Further, the plant cells of the present invention are from the Solanaceae genus Datura, especially Datura alba and Datura violet. Background technique [0002] Gastrodin (p-hydroxymethylbenzene-β-O-glucopyranoside) is the main active ingredient of Gastrodia elata, which has sedative, anti-inflammatory, anti-convulsant, analgesic and immune-enhancing effects. Gastrodin has been prepared by chemical synthesis and the synthetic gastrodin has the same biological or pharmacological activity as gastrodin from natural sources. However, the existing gastrodin synthesis process has defects such as high preparation cost, flammable raw materials, easy pollution of the environment, and poor economic benefits. Therefore, it is necessary to develop a new economical, safe, pollution-free and efficient preparation method of gastrodin. ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/04C12P19/60
Inventor 龚加顺
Owner KPC PHARM INC
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